FEI Tecnai 12 BioTWIN Transmission Electron Microscope

FEI Tecnai 12 BioTWIN 透射电子显微镜

• 批准号: 7212462
• 负责人: J. Michael MCCAFFERY
• 金额: $ 47.6万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-15 至 2008-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7212462
• 关键词: Apoptosis; Arts; Biochemical; Biological; Biology; Biopolymers; Cell Adhesion; Cells; Contract Services; Cryoultramicrotomy; Electron Microscope; Endocytosis; Endosomes; Engineering; Ensure; Funding; Future; Genes; Image; Imagery; Label; Laboratories; Lysosomes; Maintenance; Medicine; Membrane Protein Traffic; Microscopy; Microtomy; Mitochondria; Public Health; Quantum Dots; Recovery; Research Personnel; Resources; Schools; Science; Services; Sialic Acids; Simplexvirus; System; United States National Institutes of Health; Work; lens; membrane assembly; repaired; trafficking; transmission process

DESCRIPTION (provided by applicant): A diverse group of 14 NIH funded investigators, within the Johns Hopkins Schools of Arts and Sciences, Engineering, Medicine, and Public Health request funds to purchase a FEI Tecnai 12 BioTWIN transmission electron microscope (TEM). The requested TEM incorporates an objective lens configuration optimized for high contrast and is perfectly suited for our biological applications that include conventional thin section TEM; immunogold labeling of ultrathin cryosections; quantum dot; and biopolymer visualization. Importantly, this new state-of-the-art TEM will replace our well utilized near 30 year old Philips EM 410 TEM for which service (and parts) is becoming increasingly problematic; and future reliability a major concern with the system incurring extended downtimes during the previous year while parts are located to effect repair. Our investigators, all well funded through the NIH, work on a host of basic cell biological, biochemical, and biomolecular related questions including, though not limited to: HSV assembly and maturation; MHC I trafficking and assembly, membrane organization and dynamics; endosome/lysosome dynamics; membrane trafficking; trafficking of polymeric gene carriers; mitochondria and apoptosis; sialic acid display and cell adhesion; and mechanisms of endocytosis. The new Tecnai 12 will ensure that our disparate group of investigators have continued and uninterrupted access to a TEM optimized for biological applications. The Tecnai 12 will be conveniently located, administered, and maintained, in the Biology Department's newly renovated Integrated Imaging Center (IIC), a Homewood campus/Hopkins-wide microscopy resource that is utilized regularly by multiple schools and departments comprising >60 laboratories and ~200 users. It will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance/service contracts.

描述（由申请人提供）：约翰·霍普金斯大学艺术与科学学院、工程学院、医学学院和公共卫生学院内由 14 名 NIH 资助的研究人员组成的多元化小组请求资金购买 FEI Tecnai 12 BioTWIN 透射电子显微镜 (TEM)。所需的 TEM 结合了针对高对比度而优化的物镜配置，非常适合我们的生物应用，包括传统的薄切片 TEM；超薄冷冻切片的免疫金标记；量子点；和生物聚合物可视化。重要的是，这款先进的新型 TEM 将取代我们使用已近 30 年、使用良好的飞利浦 EM 410 TEM，后者的服务（和零件）问题日益严重；未来的可靠性是系统在去年因部件定位进行维修而导致停机时间延长的主要问题。我们的研究人员均通过 NIH 获得充足的资助，致力于研究一系列基本的细胞生物学、生化和生物分子相关问题，包括但不限于： HSV 组装和成熟； MHC I 运输和组装、膜组织和动力学；内体/溶酶体动力学；膜贩运；贩运聚合基因载体；线粒体和细胞凋亡；唾液酸展示和细胞粘附；和内吞作用的机制。新的 Tecnai 12 将确保我们不同的研究人员小组能够持续不间断地访问针对生物应用优化的 TEM。 Tecnai 12 将在生物系新装修的综合成像中心 (IIC) 中方便地定位、管理和维护，该中心是霍姆伍德校园/霍普金斯范围内的显微镜资源，由多个学校和部门定期使用，包括超过 60 个实验室和约 200 个用户。它将被纳入IIC现有的、完善的充值系统中，以确保回收供应品和维护/服务合同的资金。

项目成果

The assembly domain of the small capsid protein of Kaposi's sarcoma-associated herpesvirus.

卡波西肉瘤相关疱疹病毒小衣壳蛋白的组装结构域。

• DOI: 10.1128/jvi.01430-12
• 发表时间: 2012
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Kreitler,Dale;Capuano,ChristopherM;Henson,BrandonW;Pryce,ErinN;Anacker,Daniel;McCaffery,JMichael;Desai,PrashantJ
• 通讯作者: Desai,PrashantJ