Acquisition of Sony Biotechnology iCyt Synergy SY3200 BSC Cell Sorter

收购索尼生物技术 iCyt Synergy SY3200 BSC 细胞分选仪

• 批准号: 8639196
• 负责人: J. Michael MCCAFFERY
• 金额: $ 51.97万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-15 至 2015-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8639196
• 关键词: Affect; Age; Area; Arts; Baltimore; Biology; Biotechnology; Cell Separation; Cells; Chemicals; Computers; Containment; Education; Educational Curriculum; Educational workshop; Engineering; Ensure; Funding; Head; Hospitals; Image; Laboratories; Letters; Maintenance; Microscopy; Recovery; Research Personnel; Resources; Schools; Science; Site; Sorting - Cell Movement; Specimen; System; Training; Universities; design; interest; oncology; operation

DESCRIPTION (provided by applicant): 13 investigators from The Johns Hopkins University, comprising the School of Arts & Sciences (and the Department of Biology); the Whiting School of Engineering (and the Department of Chemical and Biomolecular Engineering and the PS-OC Engineering in Oncology Center); and the Union Memorial Hospital and the Orthobiologic Laboratory request $519,686 to purchase a Sony Biotechnology Synergy SY3200 Cell sorter. This system will be sited in the Johns Hopkins' Integrated Imaging Center (IIC, ://www.jhu.edu/iic); and become the centerpiece of an upgraded BSL-2 cell sorting facility serving the diverse needs of the Hopkins' Homewood Campus and North Baltimore area generally. The IIC is a Homewood campus/Hopkins-wide microscopy resource, jointly supported by the Krieger School of Arts and Sciences and the Whiting School of Engineering (see Ball & Douglas letter); and utilized regularly by multiple schools and departments comprising >150 laboratories and >450 users. The new Synergy sorter will replace a BD FACSVantage SE with DIVA option whose antiquated design and complexity makes it difficult and costly to operate/use routinely; dangerous for the operator due to bio- containment concerns during sorting that necessarily limit sorting to BL-1 level specimens; and unreliable and problematic to maintain due to its age and lack of parts availability. The new iCyt Synergy SY3200 sorter incorporates a sorting head that is integrated into a Baker SterilGARD(R) lll hood making it particularly safe for the operator with the sorter operations/adjustments being affected remotely by computer. Nozzle exchange is simple and necessary alignments automated and computer controlled. Additionally, with the sorter head integrated into the Baker BSC, the design is particularly compact. When installed, the new system will provide a BSL-2 sorting capability not currently available in North Baltimore and on the Hopkins' Homewood campus. Currently, users must wait for weeks to gain access to sorters available at the Hopkins' East Baltimore or Bay View campuses located inconveniently five and ten miles away respectively. It will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance; it will be made freely available to all interested users Hopkins-wide through workshops & hands-on training; and it will be incorporated into the Center for Biotechnology Education's Masters in Biotechnology curriculum; and the IIC's annual undergraduate/graduate course offerings. (://www.jhu.edu/iic/academic.htm).

简介（申请人提供）：来自约翰霍普金斯大学的13名研究人员，包括艺术与科学学院（和生物系）；怀廷工程学院（化学与生物分子工程系和肿瘤中心PS-OC工程）；联合纪念医院和骨科实验室要求519,686美元购买索尼生物技术协同SY3200细胞分选机。该系统将放置在约翰霍普金斯大学的综合成像中心（IIC,://www.jhu.edu/iic）；并成为升级后的BSL-2细胞分选设施的核心，服务于霍普金斯大学霍姆伍德校区和北巴尔的摩地区的各种需求。IIC是霍姆伍德校园/霍普金斯大学范围内的显微镜资源，由克里格艺术与科学学院和怀廷工程学院共同支持（见Ball和Douglas信件）；并被多个学院和部门定期使用，包括150个实验室和450个用户。新的Synergy分选机将用DIVA选项取代原有的BD facadvantage SE分选机，后者的设计陈旧且复杂，使得常规操作/使用困难且成本高昂；由于分拣过程中的生物防护问题，对操作人员有危险，必须将分拣限制在BL-1级标本；而且由于它的年代久远和缺少可用部件，维修起来也不可靠和有问题。新的iCyt Synergy SY3200分选机集成了一个分选头，该分选头集成到Baker SterilGARD(R) 11通风罩中，使其特别安全

项目成果

Differential HDAC6 Activity Modulates Ciliogenesis and Subsequent Mechanosensing of Endothelial Cells Derived from Pluripotent Stem Cells.

差异 HDAC6 活性调节纤毛发生和随后源自多能干细胞的内皮细胞的机械传感。

• DOI: 10.1016/j.celrep.2018.06.083
• 发表时间: 2018
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: Smith,Quinton;Macklin,Bria;Chan,XinYi;Jones,Hannah;Trempel,Michelle;Yoder,MervinC;Gerecht,Sharon
• 通讯作者: Gerecht,Sharon

Mimicking lichens: incorporation of yeast strains together with sucrose-secreting cyanobacteria improves survival, growth, ROS removal, and lipid production in a stable mutualistic co-culture production platform.

• DOI: 10.1186/s13068-017-0736-x
• 发表时间: 2017
• 期刊: Biotechnology for biofuels
• 影响因子: 6.3
• 作者: Li T;Li CT;Butler K;Hays SG;Guarnieri MT;Oyler GA;Betenbaugh MJ
• 通讯作者: Betenbaugh MJ

E-selectin-mediated rolling facilitates pancreatic cancer cell adhesion to hyaluronic acid.

E-选择素介导的滚动促进胰腺癌细胞与透明质酸的粘附。

• DOI: 10.1096/fj.201700331r
• 发表时间: 2017
• 期刊: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
• 作者: Shea,DanielJ;Li,YiW;Stebe,KathleenJ;Konstantopoulos,Konstantinos
• 通讯作者: Konstantopoulos,Konstantinos

Compliant substratum guides endothelial commitment from human pluripotent stem cells.

• DOI: 10.1126/sciadv.1602883
• 发表时间: 2017-05
• 期刊: Science advances
• 影响因子: 13.6
• 作者: Smith Q;Chan XY;Carmo AM;Trempel M;Saunders M;Gerecht S
• 通讯作者: Gerecht S

Histone H3 Threonine Phosphorylation Regulates Asymmetric Histone Inheritance in the Drosophila Male Germline.

• DOI: 10.1016/j.cell.2015.10.002
• 发表时间: 2015-11-05
• 期刊: Cell
• 影响因子: 64.5
• 作者: Xie J;Wooten M;Tran V;Chen BC;Pozmanter C;Simbolon C;Betzig E;Chen X
• 通讯作者: Chen X