MOLECULAR CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 分子核心的具体目标是（1）为初级COBRE研究人员提供基本的实验室服务，以支持他们以干细胞生物学为中心的基础科学研究，（2）为初级研究人员及其工作人员提供指导，监督和解决问题，进行特定的实验室检测，（3）协助指导少数核心确定的候选人。核心将保留新的实验室空间，该空间专为分子核心使用而创建。实验室配备了最先进的设备，足以进行分子生物学的所有实验。核心将免费为COBRE初级调查员提供支持，初级调查员定义为未获得独立R 01级支持的个人。核心将以补贴的服务收费方式向最近获得资助的COBRE研究者提供分析支持，并以全额收费方式向非COBRE研究者提供分析支持。Core将提供用于核酸定量和操作的常用实验室检测（PCR引物设计、真实的实时PCR定量、北方印迹检测、核糖核酸酶保护检测、非病毒/慢病毒、腺病毒表达构建体的创建）。核心将维持人类和小鼠细胞系及其相关培养基。核心将协助研究人员在蛋白质操作的各个方面，包括蛋白质印迹，免疫沉淀，标签（如His）表达构建体的创建，SILAC实验的设计。核心由在上述检测试剂盒方面具有广泛专业知识并已发表涉及这些检测试剂盒的工作的人员组成。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aims of the Molecular Core are to (1) To provide basic laboratory services to junior COBRE investigators in support of their stem cell biology centered basic science investigations, (2) To provide instruction, oversight and trouble shooting to junior investigators and their staff in the conduct of specific laboratory assays, (3) To assist in mentoring candidates identified by the Minority Core. The Core will maintain new laboratory space that has been created for the sole use of the Molecular Core. The laboratories are equipped with state-of-the art equipment sufficient for all experiments in molecular biology. The Core will offer support at no-cost to COBRE junior investigators defined as individuals who have not garnered independent R01-level support. The Core will offer assay support to recently funded COBRE investigators on a subsidized fee-for-service basis and non-COBRE investigators at a full-fee schedule. The Core will offer commonly used laboratory assays for the quantification and manipulation of nucleic acids (pcr primer design, Real Time PCR quantification, northern blot detection, ribonuclease protection assay, creation of nonviral/lentiviral, adenoviral expression constructs). The Core will maintain human and murine cell lines and their associated media. The Core will assist investigators in all aspects of protein manipulation including western blot, immunoprecipitation, creation of tagged (e.g His) expression constructs, design of SILAC experiments. The Core is staffed by individuals who have broad expertise in the above assays and who have published their work involving these assays.