MOLECULAR CORE

分子核心

• 批准号: 8168495
• 负责人: Bharat Ramratnam
• 金额: $ 32.63万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168495
• 关键词: Arts; Basic Science; Biological Assay; Cell Line; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Detection; Equipment; Fee Schedules; Fee-for-Service Plans; Funding; Grant; Human; Immunoprecipitation; Individual; Institution; Instruction; Investigation; Laboratories; Mentors; Minority; Molecular; Molecular Biology; Mus; Northern Blotting; Nucleic Acids; Proteins; Publishing; Research; Research Personnel; Resources; Ribonucleases; Services; Source; Time; United States National Institutes of Health; Western Blotting; Work; base; cost; design; design and construction; research study; stem cell biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aims of the Molecular Core are to (1) To provide basic laboratory services to junior COBRE investigators in support of their stem cell biology centered basic science investigations, (2) To provide instruction, oversight and trouble shooting to junior investigators and their staff in the conduct of specific laboratory assays, (3) To assist in mentoring candidates identified by the Minority Core. The Core will maintain new laboratory space that has been created for the sole use of the Molecular Core. The laboratories are equipped with state-of-the art equipment sufficient for all experiments in molecular biology. The Core will offer support at no-cost to COBRE junior investigators defined as individuals who have not garnered independent R01-level support. The Core will offer assay support to recently funded COBRE investigators on a subsidized fee-for-service basis and non-COBRE investigators at a full-fee schedule. The Core will offer commonly used laboratory assays for the quantification and manipulation of nucleic acids (pcr primer design, Real Time PCR quantification, northern blot detection, ribonuclease protection assay, creation of nonviral/lentiviral, adenoviral expression constructs). The Core will maintain human and murine cell lines and their associated media. The Core will assist investigators in all aspects of protein manipulation including western blot, immunoprecipitation, creation of tagged (e.g His) expression constructs, design of SILAC experiments. The Core is staffed by individuals who have broad expertise in the above assays and who have published their work involving these assays.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 分子核心的具体目标是 (1) 为初级 COBRE 研究人员提供基本实验室服务，以支持他们以干细胞生物学为中心的基础科学研究，(2) 为初级研究人员及其工作人员进行特定实验室检测提供指导、监督和故障排除，(3) 协助指导少数核心确定的候选人。核心将保留专为分子核心使用而创建的新实验室空间。实验室配备了足以进行所有分子生物学实验的最先进的设备。核心将为 COBRE 初级调查员免费提供支持，这些初级调查员被定义为尚未获得独立 R01 级支持的个人。核心将以按服务收费为基础向最近资助的 COBRE 研究人员提供检测支持，并以全额费用表向非 COBRE 研究人员提供检测支持。该核心将提供用于核酸定量和操作的常用实验室测定（PCR 引物设计、实时 PCR 定量、Northern 印迹检测、核糖核酸酶保护测定、非病毒/慢病毒、腺病毒表达构建体的创建）。核心将维护人类和小鼠细胞系及其相关培养基。 Core 将在蛋白质操作的各个方面为研究人员提供帮助，包括蛋白质印迹、免疫沉淀、标记（例如 His）表达构建体的创建、SILAC 实验的设计。核心人员由在上述检测方面拥有广泛专业知识并发表了涉及这些检测的工作的人员组成。