MOLECULAR CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分子核心的具体目标是(1)为初级Cobre研究人员提供基本实验室服务，以支持他们以干细胞生物学为中心的基础科学研究；(2)为初级研究人员及其工作人员提供指导、监督和故障排除，进行特定的实验室分析；(3)协助指导少数核心确定的候选人。核心将维护为分子核心独家使用而创建的新实验室空间。这些实验室配备了最先进的设备，足以满足分子生物学的所有实验。核心将为Cobre初级调查人员提供免费支持，这些初级调查人员被定义为没有获得独立R01级支持的个人。Core将以按服务费补贴的方式向最近资助的Cobre研究人员提供化验支持，并以全额费用时间表向非Cobre研究人员提供分析支持。Core将为核酸的定量和操作提供常用的实验室分析方法(聚合酶链式反应引物设计、实时聚合酶链式反应定量、Northern印迹检测、核糖核酸酶保护分析、创建非病毒/慢病毒、腺病毒表达结构)。核心将维持人类和小鼠的细胞系及其相关的培养液。核心将在蛋白质操作的所有方面协助研究人员，包括蛋白质印迹、免疫沉淀、标签(如His)表达结构的创建、SILAC实验的设计。核心中心的工作人员是在上述化验方面拥有广泛专业知识并发表了涉及这些化验的工作的个人。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aims of the Molecular Core are to (1) To provide basic laboratory services to junior COBRE investigators in support of their stem cell biology centered basic science investigations, (2) To provide instruction, oversight and trouble shooting to junior investigators and their staff in the conduct of specific laboratory assays, (3) To assist in mentoring candidates identified by the Minority Core. The Core will maintain new laboratory space that has been created for the sole use of the Molecular Core. The laboratories are equipped with state-of-the art equipment sufficient for all experiments in molecular biology. The Core will offer support at no-cost to COBRE junior investigators defined as individuals who have not garnered independent R01-level support. The Core will offer assay support to recently funded COBRE investigators on a subsidized fee-for-service basis and non-COBRE investigators at a full-fee schedule. The Core will offer commonly used laboratory assays for the quantification and manipulation of nucleic acids (pcr primer design, Real Time PCR quantification, northern blot detection, ribonuclease protection assay, creation of nonviral/lentiviral, adenoviral expression constructs). The Core will maintain human and murine cell lines and their associated media. The Core will assist investigators in all aspects of protein manipulation including western blot, immunoprecipitation, creation of tagged (e.g His) expression constructs, design of SILAC experiments. The Core is staffed by individuals who have broad expertise in the above assays and who have published their work involving these assays.