MOLECULAR CORE

分子核心

• 批准号: 8360132
• 负责人: Bharat Ramratnam
• 金额: $ 33.14万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360132
• 关键词: Basic Science; Biological Assay; Cell Line; Centers of Research Excellence; Clinical Research; Detection; Equipment; Fee Schedules; Fee-for-Service Plans; Funding; Grant; Human; Immunoprecipitation; Individual; Instruction; Investigation; Laboratories; Molecular; Molecular Biology; Mus; National Center for Research Resources; Northern Blotting; Nucleic Acids; Principal Investigator; Proteins; Publishing; Research; Research Infrastructure; Research Personnel; Resources; Ribonucleases; Services; Source; Time; United States National Institutes of Health; Western Blotting; Work; base; cost; design; research study; stem cell biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The specific aims of the Molecular Core are to (1) To provide basic laboratory services to junior COBRE investigators in support of their stem cell biology centered basic science investigations, (2) To provide instruction, oversight and troubleshooting to junior investigators and their staff in the conduct of specific laboratory assays, The Core will maintain new laboratory space that has been created for the sole use of the Molecular Core. The laboratories are equipped with state-of-the art equipment sufficient for all experiments in molecular biology. The Core will offer support at no-cost to COBRE junior investigators defined as individuals who have not garnered independent R01-level support. The Core will offer assay support to recently funded COBRE investigators on a subsidized fee-for-service basis and non-COBRE investigators at a full-fee schedule. The Core will offer commonly used laboratory assays for the quantification and manipulation of nucleic acids (pcr primer design, Real Time PCR quantification, northern blot detection, ribonuclease protection assay, creation of nonviral/lentiviral, adenoviral expression constructs). The Core will maintain human and murine cell lines and their associated media. The Core will assist investigators in all aspects of protein manipulation including western blot, immunoprecipitation, design of SILAC experiments. The Core is staffed by individuals who have broad expertise in the above assays and who have published their work involving these assays.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 分子核心的具体目标是（1）为初级COBRE研究人员提供基本的实验室服务，以支持他们以干细胞生物学为中心的基础科学研究，（2）为初级研究人员及其工作人员提供指导，监督和故障排除，以进行特定的实验室测定，核心将保留新的实验室空间，该空间专为分子核心使用而创建。实验室配备了最先进的设备，足以进行分子生物学的所有实验。核心将免费为COBRE初级调查员提供支持，初级调查员定义为未获得独立R 01级支持的个人。核心将以补贴的服务收费方式向最近获得资助的COBRE研究者提供分析支持，并以全额收费方式向非COBRE研究者提供分析支持。Core将提供用于核酸定量和操作的常用实验室检测（PCR引物设计、真实的实时PCR定量、北方印迹检测、核糖核酸酶保护检测、非病毒/慢病毒、腺病毒表达构建体的创建）。核心将维持人类和小鼠细胞系及其相关培养基。核心将协助研究人员在蛋白质操作的各个方面，包括蛋白质印迹，免疫沉淀，SILAC实验的设计。核心由在上述检测试剂盒方面具有广泛专业知识并已发表涉及这些检测试剂盒的工作的人员组成。