CONFOCAL MICROSCOPE: CELL BIOL, SIGNAL TRANSDUCTION, PROTEIN, CYTOPLASMIC LIPID

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：贝斯以色列女执事医疗中心 (BIDMC) 的成像核心设施请求资金购买配备倒置显微镜的 Zeiss LSM510 META 共焦系统，用于在活细胞和固定和染色组织中进行 FRET 分析，并对活细胞中的细胞内 pH 和钙进行比率成像。该仪器将放置在主动成像核心设施中，并由经验丰富的主任和高级研究助理管理。此外，还聘请了一位 FRET 技术专家为主任提供建议并与主要和次要用户组合作。 BIDMC 热情支持这项工作，并为该仪器的技术援助、空间和长期维护提供了强有力的机构承诺。主要用户组由来自 6 个不同医学组（分子医学、过敏和炎症、血液学/肿瘤学（癌症生物学计划和信号转导）和外科（普通外科））的 8 名 NIH 资助的研究人员组成。还确定了 5 名次要用户，其中 4 人在哈佛医学院附属机构的异地。提供了每组主要用户的初步数据，以显示研究的可行性，以证明使用这一新功能的合理性。仪器和组件，并展示与拟议实验相关的 FRET 和/或比率成像技术方面的专业知识，主要和次要用户组未使用的时间将被分配用于 BIDMC 核心设施中的按服务收费使用，因为目前申请机构内或朗伍德医学领域内没有类似的设备可供主要或次要用户组的成员使用。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Imaging Core Facility at Beth Israel Deaconess Medical Center (BIDMC) requests funds to purchase a Zeiss LSM510 META confocal system configured with an inverted microscope for the purpose of performing FRET analysis in living cells and fixed and stained tissues, and for performing ratio imaging of intracellular pH and calcium in living cells. The instrument will be placed in an active imaging core facility and managed by an experienced Director and Sr. Research Associate. In addition, an expert at FRET techniques was recruited to provide advice to the Director and to collaborate with the Major and Minor User Groups. The BIDMC enthusiastically supports this effort and has provided strong institutional commitment for technical assistance, space, and long-term maintenance of the instrument. The Major User Group is composed of eight NIH-funded investigators from 6 different groups in Medicine (Molecular Medicine, Allergy and Inflammation, Hematology/Oncology (Cancer Biology Program and Signal Transduction) and Surgery (General Surgery). Five Minor Users were also identified, 4 of whom are off-site at Harvard Medical School affiliated-institutions. Preliminary data from each group of major users are provided to show feasibility for the studies, to justify use of this new instrumentation and components, and to demonstrate expertise in techniques of FRET and/or of ratio imaging pertinent to the proposed experiments. Time not used by the major and minor user groups will be allotted for fee-for-service use in the BIDMC core facility. The instrumentation is requested because no like equipment is currently available to members of the major or minor user groups within the applicant institution or in the Longwood Medical area.