CONFOCAL MICROSCOPE: CELL BIOL, SIGNAL TRANSDUCTION, PROTEIN, CYTOPLASMIC LIPID

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：贝斯以色列女执事医疗中心（BIDMC）的成像核心设施需要资金购买配置有倒置显微镜的Zeiss LSM 510 Meta共聚焦系统，用于在活细胞和固定和染色组织中进行FRET分析，并用于在活细胞中进行细胞内pH和钙的比率成像。仪器将放置在有源成像核心设施中，并由经验丰富的主管和高级研究助理管理。此外，还聘请了一名FRET技术专家向主任提供咨询意见，并与主要和次要用户群体合作。BIDMC热情地支持这一努力，并为技术援助、空间和仪器的长期维护提供了强有力的机构承诺。主要用户组由来自6个不同医学组（分子医学，过敏和炎症，血液学/肿瘤学（癌症生物学计划和信号转导）和外科（普通外科））的8名NIH资助的研究人员组成。还确定了5名未成年人用户，其中4人在哈佛医学院附属机构的非现场。从每组主要用户提供的初步数据，以显示研究的可行性，以证明使用这种新的仪器和组件，并证明专业知识的FRET技术和/或比率成像相关的拟议实验。主要和次要用户组未使用的时间将分配给BIDMC核心设施的收费服务使用。要求提供仪器是因为申请机构或Longwood Medical区域内的主要或次要用户群体成员目前没有类似设备可用。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Imaging Core Facility at Beth Israel Deaconess Medical Center (BIDMC) requests funds to purchase a Zeiss LSM510 META confocal system configured with an inverted microscope for the purpose of performing FRET analysis in living cells and fixed and stained tissues, and for performing ratio imaging of intracellular pH and calcium in living cells. The instrument will be placed in an active imaging core facility and managed by an experienced Director and Sr. Research Associate. In addition, an expert at FRET techniques was recruited to provide advice to the Director and to collaborate with the Major and Minor User Groups. The BIDMC enthusiastically supports this effort and has provided strong institutional commitment for technical assistance, space, and long-term maintenance of the instrument. The Major User Group is composed of eight NIH-funded investigators from 6 different groups in Medicine (Molecular Medicine, Allergy and Inflammation, Hematology/Oncology (Cancer Biology Program and Signal Transduction) and Surgery (General Surgery). Five Minor Users were also identified, 4 of whom are off-site at Harvard Medical School affiliated-institutions. Preliminary data from each group of major users are provided to show feasibility for the studies, to justify use of this new instrumentation and components, and to demonstrate expertise in techniques of FRET and/or of ratio imaging pertinent to the proposed experiments. Time not used by the major and minor user groups will be allotted for fee-for-service use in the BIDMC core facility. The instrumentation is requested because no like equipment is currently available to members of the major or minor user groups within the applicant institution or in the Longwood Medical area.