Confocal Microscope for the BIDMC Imaging Core Facility

BIDMC 成像核心设施的共焦显微镜

• 批准号: 7047106
• 负责人: Susan J Hagen
• 金额: $ 42.75万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7047106
• 关键词: Israel; calcium; experience; hypersensitivity; inflammation; intracellular; medicine; neoplasm /cancer; oncology; schools; stainings; surgery; tissues

DESCRIPTION (provided by applicant): The Imaging Core Facility at Beth Israel Deaconess Medical Center (BIDMC) requests funds to purchase a Zeiss LSM510 META confocal system configured with an inverted microscope for the purpose of performing FRET analysis in living cells and fixed and stained tissues, and for performing ratio imaging of intracellular pH and calcium in living cells. The instrument will be placed in an active imaging core facility and managed by an experienced Director and Sr. Research Associate. In addition, an expert at FRET techniques was recruited to provide advice to the Director and to collaborate with the Major and Minor User Groups. The BIDMC enthusiastically supports this effort and has provided strong institutional commitment for technical assistance, space, and long-term maintenance of the instrument. The Major User Group is composed of eight NIH-funded investigators from 6 different groups in Medicine (Molecular Medicine, Allergy and Inflammation, Hematology/Oncology (Cancer Biology Program and Signal Transduction) and Surgery (General Surgery). Five Minor Users were also identified, 4 of whom are off-site at Harvard Medical School affiliated-institutions. Preliminary data from each group of major users are provided to show feasibility for the studies, to justify use of this new instrumentation and components, and to demonstrate expertise in techniques of FRET and/or of ratio imaging pertinent to the proposed experiments. Time not used by the major and minor user groups will be allotted for fee-for-service use in the BIDMC core facility. The instrumentation is requested because no like equipment is currently available to members of the major or minor user groups within the applicant institution or in the Longwood Medical area.

描述(由申请人提供)：贝丝以色列女执事医疗中心(BIDMC)的成像核心设施(BIDMC)请求资金购买蔡司LSM510元共焦系统，配置倒置显微镜，用于在活细胞和固定和染色的组织中进行FRET分析，并对活细胞中的细胞内pH和钙进行比率成像。该仪器将被放置在一个活跃的成像核心设施中，并由一名经验丰富的主任和高级研究助理进行管理。此外，还聘请了一名FRET技术专家向主任提供咨询意见，并与主要和次要用户群体合作。BIDMC热情支持这一努力，并为该仪器的技术援助、空间和长期维护提供了强有力的机构承诺。主要用户组由来自内科(分子医学、过敏和炎症、血液学/肿瘤学(癌症生物学计划和信号转导)和外科(普通外科))的6个不同组的8名NIH资助的研究人员组成。还发现了5名未成年人用户，其中4人不在哈佛医学院附属机构的现场。提供了来自每一组主要用户的初步数据，以显示研究的可行性，证明使用这一新仪器和部件的合理性，并展示与拟议实验相关的FRET和/或比率成像技术方面的专门知识。主要用户组和次要用户组未使用的时间将分配给BIDMC核心设施的收费服务使用。由于申请机构内或在朗伍德医疗区的主要或次要用户群体的成员目前没有类似的设备，因此要求使用该仪器。