4000 Q TRAP LC/MS/MS SHARED MASS SPECTROMETER: LIPIDOMICS

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：弗吉尼亚联邦大学有一个非常活跃和不断增长的资助研究人员的临界质量，致力于了解生物活性脂质的动态变化如何影响代谢和调节正常和病理人类细胞功能的信号通路。申请的资金用于购置一套Applied Biosystems 4000 Q TRAP（tm）LC/MS/MS系统，这是一套与液相色谱仪联用的混合三重四极杆/线性离子阱质谱仪。这种非常灵活的仪器结合了最灵敏的三重四极杆质谱仪的独特功能，具有最高容量的离子阱，可提供组成脂质体的各种分子的结构表征和定量所需的质量分辨率、灵敏度和动态响应。如本申请所述，来自7个不同部门的18名NIH资助的科学家和临床医生参与了超过45个NIH支持的研究项目，对该质谱仪有特定需求。4000 Q TRAP将作为一个共享的研究资源，为我们正在进行的研究计划提供必要的支持，并促进新的脂质鉴定，定量和表征的合作研究计划，特别强调细胞信号传导和功能脂质组学。提供这一请求的主要理由是，医疗中心校园内没有具备所需能力的质谱仪。该仪器将作为VCU的核心资源，在Sarah Spiegel教授和William M。格罗甘在脂质分析和核心资源管理方面拥有丰富的经验。Richard Moran教授将主持咨询委员会，监督该仪器的公平使用，以使受资助的研究人员和大学研究环境受益。购买这台质谱仪对于继续开发和实现脂质组学研究的全部潜力，以便更好地了解VCU医学中心校园内的正常细胞功能和人类疾病至关重要。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Virginia Commonwealth University has a very active and growing critical mass of funded investigators working to understand how dynamic changes in bioactive lipids influence metabolism and the signaling pathways that regulate normal and pathological human cellular functions. Funds are requested for an Applied Biosystems 4000 Q TRAP(tm) LC/MS/MS System, a hybrid triple quadrupole/linear ion trap mass spectrometer coupled with a liquid chromatograph. This exceptionally flexible instrument combines the unique capabilities of the most sensitive triple quadrupole mass spectrometer available with the highest capacity ion trap to provide mass resolution, sensitivity, and dynamic response needed for structural characterization and quantitation of the diverse molecules that comprise the lipidome. As described in this application, 18 NIH-funded scientists and clinicians from 7 different departments involved in over 45 NIH- supported research programs have specific needs for this mass spectrometer. The 4000 Q TRAP will be operated as a shared research resource to provide essential support for our ongoing research programs and to facilitate new collaborative research initiatives for lipid identification, quantitation, and characterization, with particular emphasis on cell signaling and functional lipidomics. Providing a principal justification for this request, there is no mass spectrometer with the required capabilities on the Medical Center campus. The instrument will be administered as a VCU core resource under the direction of Professors Sarah Spiegel and William M. Grogan, who have extensive experience in lipid analysis and administration of core resources. Prof. Richard Moran will chair the Advisory Committee overseeing equitable use of the instrument for the benefit of funded investigators and the university research environment. The purchase of this mass spectrometer is essential to continuing development and realization of the full potential of lipidomics research toward better understanding of normal cellular function and human disease on the VCU Medical Center campus.