Tissue Processing and Cell Culture COre

组织加工和细胞培养 COre

• 批准号: 7160728
• 负责人: Suzy Comhair
• 金额: $ 17.55万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7160728
• 关键词: bioinformatics; information systems; phenotype; sectioning; tissue /cell culture; tissue /cell preparation

The purpose of the Tissue Processing and Cell Culture Core (Core C) component of this PPG is to provide centralized expert handling of clinical samples obtained from human subjects by the Clinical Core B. The tissue processing and cell culture activities of Core C will convert the clinical samples into materials (airway cells, fluid, slides, etc.) required for the research proposed in all three Projects. This core will also utilize explanted lungs to culture and provide human primary smooth muscle and epithelial cells to the projects in this PPG. There are many advantages to having these activities done by a single Core facility rather than in each investigator's own laboratory. Foremost, the personnel staffing of the Core are highly experienced in performing the required procedures in a meticulous and standardized manner. In addition Core C will be responsible for ensuring that samples are properly characterized, inventoried, stored, and that data is mantained in a data base with appropriate access to PPG investigators and other cores. In this capacity, the Core will allow for each clinical sample to be utilized to its full potential in multiple Projects. This Core will interact closely with all Cores in this PPG (Core A, B and D) to maintain the extensive database required to catalog and manage the processed clinical materials and generate coherent links among experimental results obtained with these materials.

本PPG的组织处理和细胞培养核心（Core C）组件的目的是提供