Asthma Biorepository Core

哮喘生物储存库核心

• 批准号: 7982569
• 负责人: Suzy Comhair
• 金额: $ 18.31万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7982569
• 关键词: Asthma; Blood; Cataloging; Catalogs; Cell Culture Techniques; Cells; Clinical; Core Facility; Data; Data Collection; Databases; Ensure; Epithelial Cells; Equipment and supply inventories; Fibroblasts; Goals; Human; Inflammation; Internet; Laboratories; Link; Lung; Mus; Personnel Staffing; Phenotype; Principal Investigator; Procedures; Process; Reading; Reporting; Research; Research Personnel; Sampling; Slide; Smooth Muscle; Smooth Muscle Myocytes; Tissues; Work; Writing; biobank; clinical material; data management; design; experience; human subject; tissue processing

The purpose of the Asthma Biorepository Core Tissue Processing and Cell Culture Core (Core C) component of this TPPG is to provide centralized expert handling of clinical samples obtained from human subjects by the Clinical Core. The tissue processing and cell culture activities of Core C will convert the clinical samples into materials (ainway cells, fiuid, slides, etc.) required for the research proposed in all four Projects. This core will also utilize explanted lungs to culture and provide human primary smooth muscle, fibroblast and epithelial cells to the projects in this TPPG. Furthermore, the Core will also work closely with Core D regarding the culturing of mouse smooth muscle cells and tracheal epithelial cells. There are many advantages to having these activities done by a single Core facility rather than in each investigator's own laboratory. Foremost, the personnel staffing of the Core are highly experienced in performing the required procedures in a meticulous and standardized manner. In addition Core C will be responsible for ensuring that samples are properiy characterized, inventoried, stored, and that data is maintained in a data base with appropriate access to TPPG investigators and other cores. In this capacity, the Core will allow for each clinical sample to be utilized to its full potential in multiple Projects. This Core will interact closely with all Cores in this TPPG to maintain the extensive database required to catalog and manage the processed clinical materials and generate coherent links among experimental results obtained with these materials.

组织处理和细胞培养核心（Core C） 本TPPG的组成部分是对从人源性血液病患者中获得的临床样本进行集中的专家处理。 受试者的临床核心。核心C的组织加工和细胞培养活动将转化为 将临床样品转化为材料（气道细胞、液体、载玻片等）在所有四项研究中提出的研究所需的 项目该核心还将利用移植的肺来培养并提供人类初级平滑肌， 成纤维细胞和上皮细胞在这个TPPG的项目。此外，核心小组还将与 核心D关于小鼠平滑肌细胞和气管上皮细胞的培养。有很多 由一个核心机构而不是每个研究者自己完成这些活动的优势 实验室最重要的是，核心小组的工作人员在执行所需任务方面经验丰富， 程序细致、规范。此外，核心C将负责确保 样本得到适当的表征、清点和存储，数据保存在数据库中， 适当接触TPPG调查人员和其他核心人员。在这种能力下，核心将允许每个 在多个项目中充分利用临床样本。这个核心将与所有人密切互动， 此TPPG中的核心，用于维护编目和管理已处理的 临床材料，并在用这些材料获得的实验结果之间产生连贯的联系。