Tissue Processing and Cell Culture COre

组织加工和细胞培养 COre

• 批准号: 7802835
• 负责人: Suzy Comhair
• 金额: $ 19.13万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7802835
• 关键词: Asthma; Cataloging; Catalogs; Cell Culture Techniques; Cells; Clinical; Core Facility; Data; Databases; Ensure; Epithelial Cells; Equipment and supply inventories; Human; Laboratories; Link; Liquid substance; Lung; Personnel Staffing; Procedures; Process; Research; Research Personnel; Sampling; Slide; Smooth Muscle; clinical material; experience; human subject; tissue processing

The purpose of the Tissue Processing and Cell Culture Core (Core C) component of this PPG is to provide centralized expert handling of clinical samples obtained from human subjects by the Clinical Core B. The tissue processing and cell culture activities of Core C will convert the clinical samples into materials (airway cells, fluid, slides, etc.) required for the research proposed in all three Projects. This core will also utilize explanted lungs to culture and provide human primary smooth muscle and epithelial cells to the projects in this PPG. There are many advantages to having these activities done by a single Core facility rather than in each investigator's own laboratory. Foremost, the personnel staffing of the Core are highly experienced in performing the required procedures in a meticulous and standardized manner. In addition Core C will be responsible for ensuring that samples are properly characterized, inventoried, stored, and that data is mantained in a data base with appropriate access to PPG investigators and other cores. In this capacity, the Core will allow for each clinical sample to be utilized to its full potential in multiple Projects. This Core will interact closely with all Cores in this PPG (Core A, B and D) to maintain the extensive database required to catalog and manage the processed clinical materials and generate coherent links among experimental results obtained with these materials.

本PPG的组织处理和细胞培养核心（核心C）组件旨在提供 由临床核心B集中专家处理从人类受试者获得的临床样本。的 核心C的组织处理和细胞培养活动将临床样本转化为材料（气道 细胞、液体、载玻片等）这是三个项目中提出的研究所需的。该核心还将利用 移植的肺培养，并提供人类原代平滑肌和上皮细胞的项目， 这个PPG。由一个单一的核心设施来完成这些活动，而不是在 每个研究者自己的实验室。最重要的是，核心的人员配置在以下方面经验丰富： 一丝不苟，规范办理各项手续。 此外，核心C将负责确保样本得到适当表征、清点， 存储，并且数据保存在数据库中，PPG研究者和其他 丹在这种能力下，核心将允许每个临床样本充分利用其潜力， 多个项目。该核心将与本PPG中的所有核心（核心A、B和D）密切互动，以保持 对处理后的临床材料进行编目和管理，并生成 用这些材料获得的实验结果之间的连贯联系。