Cell Physiology Core

细胞生理学核心

• 批准号: 9232186
• 负责人: Suzy Comhair
• 金额: $ 24.28万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9232186
• 关键词: ARG2 gene; Air; Animal Model; Asthma; Biochemical; Bioenergetics; Cell Culture Techniques; Cell physiology; Cells; Coculture Techniques; Core Facility; Data; Data Analyses; Ensure; Epithelial Cells; Genes; Glycolysis; Goals; Human; Human Resources; In Vitro; Laboratories; Liquid substance; Lung; Measurement; Measures; Methods; Molecular; Mus; Muscle Cells; Nitrates; Nitrites; Oxygen Consumption; Personnel Staffing; Phenotype; Physiological; Physiology; Preparation; Procedures; Process; Production; Reproducibility; Research Personnel; Resources; Running; Sampling; Services; Slice; Smooth Muscle Myocytes; Standardization; System; Techniques; Training; Urea; Work; arginase; asthmatic; cost efficient; data sharing; design; experience; experimental study; genetic manipulation; in vivo; knock-down; overexpression; respiratory smooth muscle; transgene expression

ABSTRACT - CORE C The goal of the Cell Physiology core is to provide a high quality state-of-the art service for Cell culture techniques, and molecular and physiological related analyses, in cell systems as well as in human and animal models of asthma to all investigators in the PPG. In this context, the Cell Physiology Core will be defined by (1) Specific cell culture techniques: human and murine airway epithelial cells in an air-liquid interface system, human and murine smooth muscle cells, and co-cultures of airway epithelial cells in air-liquid interface and murine smooth muscle cells, and transgene expression or knockdown in airway epithelial cells in air-liquid interface and smooth muscle cells; (2) Cell physiology measurements: Bioenergetics: oxygen consumption, rate of glycolysis and lactate production, NOS/NO: nitrate and nitrite, arginase/urea and precision cut lung slices. This Core will enhance the scientific work for all projects by assuring that all cell physiology related analyses are standardized and performed in a uniform standardized manner, and by highly experienced and trained personnel. The core is run by experienced investigators who have had long experience in these techniques and have directed the laboratories for several years. They have developed or adapted several of the techniques and applied them to perform physiological studies in in-vitro and in-vivo systems. A central dedicated analytical resource will ensure high quality biochemical analyses in a cost efficient manner.

抽象-核心C 细胞生理核心的目标是为细胞培养提供高质量的最先进的服务 细胞系统以及人类和动物中的技术以及与分子和生理相关的分析 向PPG的所有研究人员介绍哮喘模型。在此背景下，细胞生理学核心将由(1)定义 特殊细胞培养技术：气液界面系统中的人和小鼠呼吸道上皮细胞， 人和小鼠平滑肌细胞以及气液界面共培养的呼吸道上皮细胞和 气液两相流中小鼠气道上皮细胞的转基因表达或敲除 (2)细胞生理学测量：生物能量学：耗氧量， 糖酵解率和乳酸生成率，一氧化氮合酶/一氧化氮：硝酸盐和亚硝酸盐，精氨酸酶/尿素和精密切割肺 切片。该核心将加强所有项目的科学工作，确保所有细胞生理学相关 分析是标准化的，并以统一的标准化方式进行，由经验丰富的 训练有素的人员。核心是由在这些方面有长期经验的经验丰富的调查人员管理的 技术，并指导了几年的实验室。他们开发或改编了几个 这些技术并应用于在体外和体内系统中进行生理学研究。一个中环 专门的分析资源将确保以具有成本效益的方式进行高质量的生化分析。