Multiphoton spectroscopic fiber probe with remote axial scanning

具有远程轴向扫描功能的多光子光谱光纤探头

• 批准号: 7295895
• 负责人: CHRIS XU
• 金额: $ 16.53万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7295895
• 关键词: Area; Biological Models; Caliber; Cancer Detection; Cancer Model; Characteristics; Classification; Clinical; Collaborations; Collection; Dependence; Depth; Detection; Development; Devices; Diagnosis; Diagnostic; Distal; Endoscopy; Environment; Fiber; Fiber Optics; Financial cost; Fluorescence; Generations; Genes; Goals; Head; Image; Invasive; Investigation; Length; Mechanics; Methodology; Methods; Motion; Numbers; Phase; Physiologic pulse; Positioning Attribute; Pulse taking; Range; Research; Resolution; Resources; Scanning; Science; Screening for cancer; Signal Transduction; Source; Spectrum Analysis; System; TP53 gene; Technology; Time; Tissues; Transgenic Mice; Uncertainty; Width; base; commercialization; concept; design; desire; improved; in vivo; innovation; instrumentation; novel; performance tests; programs; research study; tumor

DESCRIPTION (provided by applicant): The goal of this research program is to develop a flexible, compact, and passive fiber optic probe that is capable of axially scanning the tissue layers for cancer detection and diagnostic, and then demonstrate the value of the proposed sources for in vivo biomedical spectroscopy and endoscopy. The innovation of the proposed fiber optic probe is based on the new concept of simultaneous spatial and temporal focusing (SSTF) that was recently demonstrated in multiphoton imaging, where, in addition to conventional spatial focusing, an extra degree of axial confinement is achieved by temporally focusing an ultrashort pulse. We show that by adjusting the spectral phase of the input pulse at the proximal end of the fiber probe, we can scan the focal plane in an SSTF setup at the distal end remotely. Thus, a passive, ultracompact fiber probe can be achieved that provides high resolution z-sectioning of multiphoton excited fluorescence and/or harmonic generation over a scan depth of hundreds of microns. Leveraging the latest theoretical and experimental development of SSTF, and taking full advantage of the recent advancement of large mode area fiber for pulse delivery and signal collection, the proposed research program aims to create a practical and robust fiber optic probe that is tailored specifically for cancer detection and diagnostics. This research program involves close collaboration between the PI and Co-PI (Dr. Zipfel). The combined expertise of the research team includes all of the science and technologies required for conducting the proposed research. The proposed program, if successfully completed, leads to a novel fiber optic probe for early cancer detection and diagnosis. The advantage of multiphoton excitation through the flexible fiber probe, combined with the remote axial scanning capability, allows depth resolved signal excitation and detection, and can provide significantly improved diagnostic signature for non- invasive early cancer detection. Furthermore, the proposed fiber probe for multiphoton endoscopic spectroscopy is highly practical in a clinical environment, providing the possibility of rapid commercialization and having an immediate real-world impact.

描述（由申请人提供）：本研究计划的目标是开发一种灵活、紧凑和无源的光纤探头，能够轴向扫描组织层进行癌症检测和诊断，然后证明所提出的体内生物医学光谱学和内窥镜检查源的价值。所提出的光纤探头的创新是基于最近在多光子成像中证明的同时空间和时间聚焦（SSTF）的新概念，其中，除了传统的空间聚焦之外，通过时间聚焦超短脉冲来实现额外程度的轴向限制。我们表明，通过调整的光谱相位的输入脉冲在近端的光纤探头，我们可以扫描焦平面的SSTF设置在远端远程。因此，可以实现无源超紧凑光纤探针，其在数百微米的扫描深度上提供多光子激发荧光和/或谐波产生的高分辨率z截面。利用SSTF的最新理论和实验发展，并充分利用大模场光纤用于脉冲传输和信号收集的最新进展，拟议的研究计划旨在创建一种实用而强大的光纤探头，专门用于癌症检测和诊断。本研究计划涉及PI和Co-PI（Zipfel博士）之间的密切合作。研究团队的综合专业知识包括开展拟议研究所需的所有科学和技术。该计划如果成功完成，将导致一种用于早期癌症检测和诊断的新型光纤探针。通过柔性光纤探针的多光子激发的优点与远程轴向扫描能力相结合，允许深度分辨信号激发和检测，并且可以为非侵入性早期癌症检测提供显著改进的诊断特征。此外，所提出的用于多光子内窥镜光谱学的光纤探头在临床环境中非常实用，提供了快速商业化的可能性，并具有直接的现实影响。