Neuroimmune Control of Biobahavioral Processes

生物行为过程的神经免疫控制

• 批准号: 7173371
• 负责人: Rodney W Johnson
• 金额: $ 29.51万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7173371
• 关键词: Admission activity; Adolescent; Affect; Age; Age-Years; Aging; Animals; Anti-Inflammatory Agents; Anti-inflammatory; Antibodies; Attenuated; Behavior; Behavior Therapy; Behavioral; Blood; Brain; Cardiovascular system; Chronic; Clinical; Clinical Research; Cognition; Cognitive; Cognitive deficits; Condition; Coupled; Craniocerebral Trauma; Daily; Data; Dementia; Development; Elderly; Elevation; Equilibrium; Etiology; Event; Fatigue; Genetically Engineered Mouse; Growth Factor; Hippocampus (Brain); Hormones; Hospital Nursing; Housing; Human; Impaired cognition; In Vitro; Independent Living; Individual; Injection of therapeutic agent; Insulin-Like Growth Factor I; Interleukin-1; Interleukin-10; Interleukin-6; Investigation; Knockout Mice; Laboratories; Lead; Life; Living Wills; Measurement; Measures; Mediating; Memory; Memory Loss; Modeling; Molecular; Mood Disorders; Motivation; Motor; Motor Activity; Mus; Muscle; NF-kappa B; Neurodegenerative Disorders; Neurons; Nursing Homes; Parkinson Disease; Patient Self-Report; Peptides; Performance; Personal Satisfaction; Physiology; Plasma; Process; Protein Biosynthesis; Proteins; Proto-Oncogene Proteins c-akt; Psychomotor Performance; Psychoneuroimmunology; Puberty; Rate; Research Personnel; Research Project Grants; Resistance; Rodent; Running; Symptoms; TNF gene; TNFRSF5 gene; Testing; Therapeutic Intervention; Thinking; Upper arm; Walking; Withdrawal; age related; aged; base; biobehavior; cell type; concept; cytokine; day; experience; falls; germ free condition; healthy aging; immune function; in vitro Model; in vivo; indexing; innovation; juvenile animal; mature animal; normal aging; novel strategies; programs; research study; retinal rods; skills; social; toll-like receptor 4; wasting

Very little is known about chronic, long-term actions of endogenous proinflammatory cytokines on basic biobehavioral processes, particularly neuroimmune-mediated sickness behavior, motor function, fatigue and memory. We and others have documented a significant rise in the proinflammatory cytokines IL-6, IL-1 and TNF in both the periphery and brain of healthy aging mice. We hypothesize that this chronic elevation in proinflammatory cytokines is responsible for performance deficits in multiple biobehavioral processes in aged animals. We have established that IL-6 increases and IL-10 decreases significantly in the brain and plasma of healthy aging mice. These changes are associated with reduced hippocampal-dependent spatial memory and motor function (as assessed by locomotor activity and treadmill running). We have also shown that two important aspects of sickness behavior, immobility and social investigation following i.c.v, injections of either LPS or IL-1, are ameliorated in IL-6 knockout mice. We postulate that the normal, chronic, age-associated rise in IL-6, IL-1 and TNF impacts numerous biobehavioral processes, as assessed by an increase in sickness-related behaviors (diminished social investigation, spatial memory loss) and a reduction in motor function (fatigue on a treadmill, alternations in a 4-arm plus maze, stationery rod). In Objective 1, we will test the hypothesis that all of these age-associated biobehavioral variables are attenuated in Toll-like receptor-4 and NF-KappaB p50 knockout mice but are exacerbated in mice deficient in IL-10. Objective 2 will use IL-6- deficient mice and cytokine-specific antibodies to define intracellular substrates that lead to age-associated reductions in these biobehavioral events. Objective 3 will expand our exciting findings that IGF-I, a growth factor that behaves as an anti-inflammatory cytokine and declines during aging, counteracts age-associated biobehavioral deficits. Finally, Objective 4 will utilize a well-defined model that is very likely to explain the molecular events that are directly responsible for biobehavioral performance deficits in both muscle and brain. These experiments will use both in vitro and in vivo approaches and are needed to understand how chronic, long-term elevations in proinflammatory cytokines impair important biobehavioral processes.

关于内源性促炎细胞因子在基本生物行为过程中的长期慢性作用，尤其是神经免疫介导的疾病行为、运动功能、疲劳和记忆，人们知之甚少。我们和其他人已经证明，在健康衰老的小鼠的外周和大脑中，促炎细胞因子IL-6、IL-1和肿瘤坏死因子显著增加。我们假设，这种促炎细胞因子的慢性升高是老年动物在多种生物行为过程中表现缺陷的原因。我们已经证实，在健康衰老小鼠的脑和血浆中，IL-6显著增加，而IL-10显著降低。这些变化与依赖海马体的空间记忆和运动功能(通过运动活动和跑步机跑步来评估)减少有关。我们还表明，在IL-6基因敲除小鼠中，疾病行为的两个重要方面，即静坐不动和静脉注射内毒素或IL-1后的社会调查，都得到了改善。我们推测，正常的、慢性的、与年龄相关的IL-6、IL-1和TNF的升高影响了许多生物行为过程，如疾病相关行为的增加(减少的社会调查、空间记忆丧失)和运动功能的降低(跑步机上的疲劳、四臂加迷宫的交替、文具棒)。在目标1中，我们将测试假设，所有这些年龄相关的生物行为变量在Toll样受体-4和NF-kappaB p50基因敲除小鼠中减弱，但在IL-10缺乏的小鼠中加剧。目标2将使用IL-6缺陷小鼠和细胞因子特异性抗体来确定导致这些生物行为事件与年龄相关的减少的细胞内底物。目标3将扩展我们令人兴奋的发现，即IGF-I，一种作为抗炎细胞因子的生长因子，在衰老过程中下降，抵消与年龄相关的生物行为缺陷。最后，目标4将利用一个定义明确的模型，该模型很可能解释直接导致肌肉和大脑生物行为表现缺陷的分子事件。这些实验将使用体外和体内的方法，需要了解促炎细胞因子的长期慢性升高如何损害重要的生物行为过程。