Biofilm Growth on Functionalized Surfaces

功能化表面上的生物膜生长

• 批准号: 7580106
• 负责人: JUN F LIANG
• 金额: $ 23.16万
• 依托单位: STEVENS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-15 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7580106
• 关键词: Accounting; Adopted; Affect; Antibiotic Resistance; Antibiotic Therapy; Antibiotics; Antimicrobial Resistance; Architecture; Bacteria; Bacterial Adhesion; Biocompatible Materials; Blood; Body Fluids; Cause of Death; Cell surface; Cells; Characteristics; Charge; Data; Development; Diagnostic; Disease; Drops; Environment; Event; Excision; Exhibits; Figs - dietary; Goals; Growth; Heart Diseases; Immune system; Implant; Infection; Left; Life; Liquid substance; Malignant Neoplasms; Mathematics; Medical; Methods; Microbial Biofilms; Modeling; Modification; Molecular; Molecular Conformation; Montana; Morbidity - disease rate; Morphology; Nosocomial Infections; Operative Surgical Procedures; Phenotype; Physiological; Polymers; Prevention; Procedures; Prosthesis; Research; Resistance development; Solid; Stroke; Structure; Surface; Testing; Time; United States; Universities; antimicrobial drug; aqueous; cell killing; cost; design; design and construction; experience; fight against; implantable device; killings; microbial; prevent; public health relevance; success; web site

DESCRIPTION (provided by applicant): Bacteria in biofilm show unique physiological characteristics that are much different from planktonic cultured phenotypes. One of the most important features of bacterial biofilms is their resistance to antimicrobial agents and the host immune system attacks. Bacteria living in biofilms can exhibit up to 1,000 time greater resistance to antibiotics than planktonic bacteria. Biofilm associated nosocomial (hospital acquired) infection and disease is currently the fourth leading cause of death in the United States, behind only heart disease, cancer and stroke. Regardless what anti-biofilm methods are used, the success rates are very limited and biofilms will nevertheless form on implanted devices. In most cases, biofilm related infections can only be cured by a high cost and undesirable procedure through the removal of the implants. In this project, we propose to fight against biofilm from a new direction by directly dealing with attached bacteria and formed biofilms. We propose to construct functional surfaces which can interrupt the formation of biofilm architecture. Our hypothesis is that biofilms formed on these functional surfaces with damaged biofilm architectures may not be able to develop antibiotic resistance. Bacteria in such biofilms may maintain antibiotic sensitivity and can be killed by ordinary antibiotic treatment. Three board objectives of this project are to 1) construct surfaces with desired structures and functions; 2) study biofilm formation dynamics and morphologies on functionalized surfaces; 3) test the antibiotic sensitivity of "biofilms" on functionalized surfaces and study sensitizing mechanisms using different antibiotic treatment approaches and fitting data with established mathematic models. PUBLIC HEALTH RELEVANCE: Biofilm Growth on Functionalized Surfaces Narrative Bacteria can attach to the surfaces of implant devices and grow into bacteria clusters (called biofilms). Unlike free bacteria, bacterial in biofilms can hardly be killed by ordinary antibiotic treatment. Biofilm associated infection and disease is currently the fourth leading cause of death in the United States, behind only heart disease, cancer, and stroke. The goal of this research is to develop new types of surfaces to prevent biofilm related infections and diseases.

描述（由申请人提供）：生物膜中的细菌显示出独特的生理特征，与体外培养的表型有很大不同。细菌生物膜最重要的特征之一是它们对抗菌剂和宿主免疫系统攻击的抗性。生活在生物膜中的细菌对抗生素的耐药性比嗜热菌高出1,000倍。生物膜相关的医院（医院获得性）感染和疾病目前是美国第四大死亡原因，仅次于心脏病，癌症和中风。无论使用何种抗生物膜方法，成功率都非常有限，而且生物膜仍将在植入器械上形成。在大多数情况下，生物膜相关的感染只能通过移除植入物的高成本和不期望的程序来治愈。在本项目中，我们提出从一个新的方向对抗生物膜，直接处理附着的细菌和形成的生物膜。我们建议构建功能表面，可以中断生物膜结构的形成。我们的假设是，在这些生物膜结构受损的功能表面上形成的生物膜可能无法产生抗生素耐药性。这种生物膜中的细菌可以保持抗生素敏感性，并且可以通过普通抗生素治疗杀死。该项目的三个目标是：1）构建具有所需结构和功能的表面; 2）研究功能化表面上生物膜形成动力学和形态; 3）测试功能化表面上“生物膜”的抗生素敏感性，并使用不同的抗生素处理方法和建立的数学模型拟合数据来研究敏化机制。公共卫生关系：功能化表面上的生物膜生长叙述细菌可以附着在植入器械的表面并生长成细菌簇（称为生物膜）。与游离细菌不同，生物膜中的细菌很难被普通的抗生素治疗杀死。生物膜相关的感染和疾病目前是美国第四大死亡原因，仅次于心脏病，癌症和中风。这项研究的目标是开发新型表面，以防止生物膜相关的感染和疾病。