RHESUS MACAQUE HEMATOPOIETIC STEM CELL EXPANSION

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。开展恒河猴胚胎干细胞向造血干细胞分化潜能的初步研究。 在过去的二十年里，造血干细胞（HSC）已用于骨髓移植来治疗多种血液恶性肿瘤。目前造血干细胞移植面临的主要问题之一是缺乏合适的造血干细胞供体。胚胎干细胞（Embryonic stem cells，ESCs）是一种潜在的造血干细胞来源。非人灵长类动物已被证明是非常宝贵的临床前模型HSC移植研究。我们正计划在体外和体内建立恒河猴胚胎干细胞向造血干细胞分化的模型。我们目前正处于研究的体外阶段。我们正在通过WNRPC接收恒河猴动物的外周血和骨髓样本，并将其用作对照和优化研究。这项研究使用了WNPRC干细胞资源和动物服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To initiate pilot studies on the differentiation potential of rhesus macaque embryonic stem cells into hematopoietic stem cells. Hematopoietic stem cells (HSCs) have been used in bone marrow transplantation for treatment of a variety of hematological malignancies for the last two decades. One of the major problems in HSC transplantation is lack of suitable HSC donors. Embryonic stem cells (ESCs) have been proposed as a potentially novel source of HSCs in the future. Nonhuman primates have been proven to be invaluable as a preclinical model for HSC transplantation research. We are planning to develop in vitro and in vivo models of differentiation of rhesus ESCs into HSCs. We are currently at the in vitro phase of our studies. We are receiving peripheral blood and bone marrows samples from rhesus animals through WNRPC and use them as controls and for optimization studies. This research used WNPRC Stem Cell Resources and Animal Services.