Biology of the Primate Ovarian Surface Epithelium

灵长类动物卵巢表面上皮的生物学

• 批准号: 7357490
• 负责人: Jay W Wright
• 金额: $ 34.15万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2011-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357490
• 关键词: Adhesions; Antibodies; Apoptosis; Apoptotic; Applied Research; Archives; Biological; Biology; Body of uterus; Cell Cycle; Cell Cycle Progression; Cells; Cessation of life; Characteristics; Collaborations; Collecting Cell; Condition; Data; Domestic Animals; Endocrine; Environment; Epithelium; Estradiol; Estrogen Receptors; Estrogens; Excision; Exposure to; Extracellular Matrix; Functional disorder; Future; Gene Expression; Goals; Growth; Health; Hormonal; Human; Infertility; Laboratories; Length; Life; Macaca; Macaca mulatta; Maintenance; Malignant - descriptor; Malignant Neoplasms; Malignant neoplasm of ovary; Mediating; Menstrual cycle; Modeling; Molecular; Numbers; Ovarian; Ovarian Cycles; Ovarian Mass; Ovary; Ovulation; Pathologic; Pathology; Phase; Population Dynamics; Prevention; Primates; Production; Progesterone; Progesterone Receptors; Protocols documentation; Recovery; Regulation; Research Personnel; Risk; Role; Serum; Site; Steroids; Structure; Surface; System; Techniques; Testing; Time; Tissues; Woman; Wound Healing; base; corpus luteum; design; indexing; insight; intraovarian; intraperitoneal; prevent; repaired; research study; restoration; size; steroid hormone; tumorigenesis

The ovarian surface epithelium (OSE) is a monolayerthat normally undergoes repair and regrowth after each ovulation during natural menstrual cycles. Although it comprises less than 1/1,000th of the entire ovarian mass, it gives rise to nearly 90% of human ovarian cancers. Little is known about the characteristics and regulation of the OSE during the ovarian cycle, or its biological role(s). The long-term objective of this project is to understand the role of the OSE in ovarian biology and how its activities and regulation contribute to normal and pathologic situations in primates. Based on studies of OSE cells collected from rhesus monkeys, which share many characteristics with human OSE cells, experiments are proposed to test the hypotheses that the primate OSE: (a) is comprised of a dynamic population of cells that undergo proliferation, differentiation, and apoptosis as a function of proximity to dominant ovarian structures (i.e.the follicle destined to ovulate and the corpus luteum) and/or as a result of their exposure to the endocrine products of these structures (i.e.estrogen and progesterone), and (b) is essential for normal ovarian activities, notably timely ovulation of the dominant follicle. Experiments in rhesus macaques are designed to: Specific Aim #1: determine the surface distribution and characteristics, including proliferative and apoptotic indices, of the OSE throughout the menstrual cycle; Specific Aim #2: examine the effects of the dominant ovarian structures (the preovulatory follicle and corpus luteum), and steroid hormones (estradiol and progesterone) on OSE proliferation and death,and; Specific Aim #3: delineate the necessity of the OSE for ovarian function, including ovulation, repair, and prevention of intraperitoneal adhesions. This project will employ for the first time molecular and cellular techniques to examine gene expression, proliferation, and apoptosis in the primate OSE, and will rigorously evaluate its role in ovarian function during the menstrual cycle. Detailed information will accrue that provides a basis for further considerations of mechanisms leading to ovarian dysfunction (e.g. infertility) and pathology (e.g. tumorigenesis). The result of this project will generate a comprehensive tissue archive to be used in future studies and collaborations with other investigators.

卵巢表面上皮(OSE)是一种单层，通常经过修复和再生后 自然月经周期中的每一次排卵。尽管它不到整个地球的千分之一 卵巢肿块是导致近90%的人类卵巢癌的原因。人们对其特征知之甚少。 卵巢周期中卵泡刺激素的调节，或其生物学作用(S)。这样做的长期目标是 项目是了解OSE在卵巢生物学中的作用以及它的活动和调节如何起作用 灵长类动物的正常和病态情况。基于对从恒河猴采集的OSE细胞的研究 猴子与人类的OSE细胞有许多共同的特征，有人提议用实验来测试 灵长类OSE：(A)由动态的细胞群体组成的假设 增殖、分化和凋亡作为接近优势卵巢结构(即 注定要排卵的卵泡和黄体)和/或由于它们暴露在内分泌 这些结构的产物(即雌激素和孕激素)，以及(B)对于正常的卵巢是必不可少的 活动，特别是优势卵泡的及时排卵。在恒河猴身上进行的实验旨在： 具体目标#1：确定表面分布和特征，包括增殖和凋亡 月经周期中的OSE指数； 具体目标#2：检查主要卵巢结构(排卵前卵泡和卵泡)的影响 黄体)和类固醇激素(雌二醇和孕酮)对OSE增殖和死亡的影响，以及； 具体目标#3：描述OSE对卵巢功能的必要性，包括排卵、修复和 预防腹膜内粘连。 该项目将首次使用分子和细胞技术来检测基因表达， 灵长类OSE的增殖和凋亡，并将严格评估其在卵巢功能中的作用。 月经周期。将提供详细的信息，为进一步审议 导致卵巢功能障碍(如不孕症)和病理(如肿瘤发生)的机制。其结果是 该项目将生成一个全面的组织档案，用于未来的研究和与 其他调查人员。