BIOLOGY OF THE PRIMATE OVARIAN SURFACE EPITHELIUM

灵长类动物卵巢表面上皮的生物学

• 批准号: 7958496
• 负责人: Jay W Wright
• 金额: $ 8.03万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-04 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958496
• 关键词: Animal Model; Biological Models; Biology; Chickens; Computer Retrieval of Information on Scientific Projects Database; Epithelial ovarian cancer; Epithelium; Estrogens; Ethics; Etiology; Funding; Gene Expression; Grant; Health; Human; Institution; Macaca mulatta; Malignant neoplasm of ovary; Mammals; Menstrual cycle; Methods; Modeling; Ovarian; Ovary; Phase; Physiology; Primates; Progesterone; Reporting; Research; Research Personnel; Resources; Risk; Source; Surface; System; United States National Institutes of Health; Woman; aged; base; human tissue; in vivo; nonhuman primate; novel therapeutics; reproductive; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The etiology of epithelial ovarian cancer is poorly understood, in part because its uniqueness to primates (and chickens) limits the availability of an animal model. In addition, the biology of the normal primate ovarian surface epithelium (OSE) has not been effectively studied, so clues to the causes of its transformation are not based on empirical observation. Due to the difficulty in obtaining appropriate human tissue (healthy ovaries from reproductively aged women) and the practical and ethical limitations on human studies, we selected the nonhuman primate rhesus monkey as a surrogate model for the human system on the basis of: (1) shared reproductive and ovarian physiology; (2) shared gene expression that is not common to nonprimate mammals; and (3) reports of epithelial ovarian cancer in human and nonhuman primates, but not other mammals, that emphasize the potential uniqueness of the primate OSE. Our aims are to characterize the primate OSE at distinct phases of the menstrual cycle using immunohistochemical methods; to determine whether ovarian factors (estrogen and progesterone) dynamically regulate the OSE in vivo; and to establish whether the OSE is an essential component of ovarian function and health. This project will establish a fundamental baseline to understand the primate OSE and provide a model system for hypothesis-based experiments to evaluate risks for ovarian cancer and novel therapeutic strategies.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 上皮卵巢癌的病因鲜为人知，部分原因是它对灵长类动物（和鸡）的独特性限制了动物模型的可用性。 此外，尚未有效研究正常灵长类动物卵巢表面上皮（OSE）的生物学，因此其转化原因的线索不是基于经验观察。 由于难以获得适当的人体组织（来自生殖老年妇女的健康卵巢）以及人类研究的实际和伦理限制，我们根据以下基础选择了非人类灵长类动物的恒河猴作为人类系统的代孕模型。 （2）共享基因表达，这对于非哺乳动物而言并不常见； （3）人类和非人类灵长类动物的上皮卵巢癌的报道，而不是其他哺乳动物，这些报道强调了灵长类动物的潜在独特性。 我们的目的是使用免疫组织化学方法在月经周期的不同阶段表征灵长类动物。确定卵巢因子（雌激素和孕酮）是否会动态调节体内OSE；并确定ose是否是卵巢功能和健康的重要组成部分。 该项目将建立一个基本的基准，以了解灵长类动物的OSE，并为基于假设的实验提供模型系统，以评估卵巢癌和新型治疗策略的风险。