BIOLOGY OF THE PRIMATE OVARIAN SURFACE EPITHELIUM

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 上皮性卵巢癌的病因学知之甚少，部分原因是其对灵长类（和鸡）的独特性限制了动物模型的可用性。 此外，正常灵长类动物卵巢表面上皮（OSE）的生物学尚未得到有效研究，因此其转化原因的线索并不基于经验观察。 由于难以获得合适的人体组织（健康的卵巢来自育龄妇女）和人类研究的实际和伦理限制，我们选择非人灵长类恒河猴作为人类系统的替代模型，其基础是：（1）共同的生殖和卵巢生理学;（2）共同的基因表达在非灵长类哺乳动物中不常见;和（3）在人类和非人类灵长类动物中，但不是在其他哺乳动物中，上皮性卵巢癌的报告，强调了灵长类OSE的潜在独特性。 我们的目的是表征灵长类动物OSE在月经周期的不同阶段，使用免疫组化方法，以确定是否卵巢因素（雌激素和孕激素）动态调节OSE在体内，并建立OSE是否是卵巢功能和健康的重要组成部分。 该项目将建立一个基本的基线，以了解灵长类动物OSE，并提供一个基于假设的实验模型系统，以评估卵巢癌的风险和新的治疗策略。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The etiology of epithelial ovarian cancer is poorly understood, in part because its uniqueness to primates (and chickens) limits the availability of an animal model. In addition, the biology of the normal primate ovarian surface epithelium (OSE) has not been effectively studied, so clues to the causes of its transformation are not based on empirical observation. Due to the difficulty in obtaining appropriate human tissue (healthy ovaries from reproductively aged women) and the practical and ethical limitations on human studies, we selected the nonhuman primate rhesus monkey as a surrogate model for the human system on the basis of: (1) shared reproductive and ovarian physiology; (2) shared gene expression that is not common to nonprimate mammals; and (3) reports of epithelial ovarian cancer in human and nonhuman primates, but not other mammals, that emphasize the potential uniqueness of the primate OSE. Our aims are to characterize the primate OSE at distinct phases of the menstrual cycle using immunohistochemical methods; to determine whether ovarian factors (estrogen and progesterone) dynamically regulate the OSE in vivo; and to establish whether the OSE is an essential component of ovarian function and health. This project will establish a fundamental baseline to understand the primate OSE and provide a model system for hypothesis-based experiments to evaluate risks for ovarian cancer and novel therapeutic strategies.