NANOSCALE FLUCTUATIONS OF ERYTHROCYTE SUBDOMAINS IMAGED BY FOURIER PHASE MICROS

傅里叶相显微镜成像的红细胞亚域的纳米级波动

• 批准号: 7357948
• 负责人: Gabriela K Popescu
• 金额: $ 4.07万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357948
• 关键词: erythrocytes; microscopy; motivation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Fourier Phase Microscopy (FPM) is a novel imaging technique that provides quantitative information about the optical path-length shifts through dynamic biological samples with sub-nanometer sensitivity over extended periods of time. FPM was used to measure nanoscale fluctuations associated with the membrane of live erythrocytes during time intervals ranging from a few seconds to hours. The experimental results demonstrate the existence of dynamic subdomains within the cell, which exhibit oscillations at various frequencies. The non-random nature of these fluctuations suggests that they are driven by deterministic phenomena associated with this living system.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。傅立叶位相显微镜(FPM)是一种新型的成像技术，它以亚纳米级的灵敏度提供了动态生物样品在长时间内光程-长度漂移的定量信息。FPM被用来测量在从几秒到几个小时的时间间隔内与活红细胞膜相关的纳米级波动。实验结果表明，细胞内存在动态的子区，在不同的频率下表现出振荡。这些波动的非随机性表明，它们是由与这个生命系统相关的确定性现象驱动的。