Practical Proteomics: A training course for the BBSRC community

从广义上讲，蛋白质组学是对大量蛋白质的鉴定和表征，目的是通过揭示蛋白质水平和活性的变化来理解生物系统。由于蛋白质组学是一个相对较新的研究领域，目前在所有专业水平的生物和生化科学的许多领域的研究人员对现代蛋白质组学技术的实践培训有很大的需求。蛋白质组学中使用的不同技术非常多样化，要求生物科学家在他们不熟悉的新领域和具有挑战性的领域成为专家。例如，质谱技术绝对是蛋白质组学研究的核心，但很少有生物学家有使用它的经验。如果使用蛋白质组学为生物科学提供的研究优势能够被英国科学充分利用，这些技术需要被英国的生物科学家更广泛和有效地使用。因此，他们需要接受培训，学会使用质谱等技术，并学习如何将蛋白质组学技术应用于他们自己的研究领域。在蛋白质组学技术方面提供必要培训的最好方法是让人们有机会在实验室中实践和学习。有了这种实践经验，生物学家将能够回到他们自己的实验室，并开始在他们自己的研究项目中有效地应用蛋白质组学。为了满足对蛋白质组学培训的需求，我们将为生物科学领域的研究人员提供一门课程，教授他们蛋白质组学技术的基础知识，并让他们有机会在实验室中实践这些技术。这门实践性强的课程为期五天，包括课堂授课、示范和实验练习。除了课堂讲授蛋白质组学的理论和实践外，课程参与者还将有机会与蛋白质组学经验丰富的科学家讨论适当的蛋白质组学实验设计。该课程将由技术专家讲授，他们在自己的研究中经常使用这些技术。学生将在课程中使用的设备和仪器是目前蛋白质组学研究中最先进和最好的。学生将从头到尾学习如何进行蛋白质组学研究，甚至可以在课程中携带自己的生物样本进行分析。我们所说的学生是指任何对蛋白质组学感兴趣且至少拥有生物或化学科学学士学位的人，包括研究生、博士后研究人员、研究人员和学术人员（讲师、读者和教授）。有了在课程中获得的知识和经验，生物科学家将能够开始在他们自己的研究中使用蛋白质组学技术。

Proteomics, broadly speaking, is the identification and characterisation of large numbers of proteins, for the purpose of understanding biological systems by uncovering changes in protein levels and activites. Because proteomics is a relatively new field of study, there is currently a great demand amongst researchers in many areas of the biological and biochemical sciences at all professional levels for hands-on training in modern proteomic technologies. The different technologies used in proteomics are very diverse and require bioscientists to become expert in new and challenging areas with which they are not familiar. For example, the technique of mass spectrometry is absolutely central to proteomics research, but few biological scientists have any experience with it. If the research advantages that using proteomics offers to the biosciences are to be properly and fully available to British science, these techniques need to be used much more widely and effectively by biological scientists in the UK. They therefore need to be trained to use techniques like mass spectrometry and be taught how proteomics technologies can be applied to their own areas of research. The best way to provide the necessary training in proteomics technologies is to give people the opportunity to practice and learn them in the laboratory. With this kind of hands-on experience, biologists will be able to return to their own laboratories and begin to apply proteomics effectively in their own research programmes. We will meet the need for training in proteomics by providing a course to researchers in the biological sciences which will teach them the basics of proteomic technologies and give them the chance to practice the technologies themselves in the laboratory. This practical, hands-on course will be five days long, and combine class-room lectures, demonstrations, and laboratory exercises. As well as class-room lectures on the theory and practice of proteomics, participants in the course will have the opportunity to discuss the design of appropriate proteomics experiments with scientists who are experienced in proteomics. The course will be taught by experts in the technologies, who are experienced at using them routinely in their own research. The equipment and instruments that will be used by the students on the course is state-of-the-art and the best that is currently available for proteomic research. The students will learn how how to carry out a proteomic study from beginning to end, and will even be able to bring their own biological samples to analyze during the course. By students, we mean anyone interested in proteomics with at least a first degree in the biological or chemical sciences, including post-graduates, post-doctoral researchers, and research and academic staff (lecturers, readers, and professors). With the knowledge and experience gained in the course, biological scientists will be able to start using proteomic technologies in their own research.