MEASUREMENT OF OXIDATIVE STRESSES IN CELLS AND TISSUES

细胞和组织中氧化应激的测量

• 批准号: 7365615
• 负责人: Bruce J. Tromberg
• 金额: $ 0.39万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7365615
• 关键词: MEASUREMENT; OXIDATIVE; STRESSES; CELLS; TISSUES

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Utilize tissue engineering technology and an in vitro model of the airway mucosa to understand the mechanisms of injury and healing of the bronchial epithelium following exposure to inhaled toxic chemical agents that include mustard gas (phosgene), chlorine gas, and 100% oxygen. Understanding of the mechanism of injury and healing will then lead to the development of therapeutic interventions. Our initial means of studying this phenomenon is to understand tissue breakdown of collagen using SHG, and to monitor oxidative stress in the cell by following fluorescence of flavins and NADH.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。利用组织工程技术和气道粘膜的体外模型，了解暴露于吸入有毒化学试剂（包括芥子气（光气），氯气和100%氧气）后支气管上皮的损伤和愈合机制。了解损伤和愈合的机制将促进治疗干预措施的发展。我们研究这种现象的最初方法是使用SHG来了解胶原蛋白的组织分解，并通过跟踪黄素和NADH的荧光来监测细胞中的氧化应激。