MEASUREMENT OF OXIDATIVE STRESSES IN CELLS AND TISSUES

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。利用组织工程技术和呼吸道粘膜的体外模型，了解吸入有毒化学物质，包括芥子气(光气)、氯气和100%氧气后，支气管上皮损伤和愈合的机制。对损伤和愈合机制的了解将导致治疗干预措施的发展。我们研究这一现象的最初方法是使用SHG了解胶原的组织分解，并通过跟踪黄素和NADH的荧光来监测细胞中的氧化应激。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Utilize tissue engineering technology and an in vitro model of the airway mucosa to understand the mechanisms of injury and healing of the bronchial epithelium following exposure to inhaled toxic chemical agents that include mustard gas (phosgene), chlorine gas, and 100% oxygen. Understanding of the mechanism of injury and healing will then lead to the development of therapeutic interventions. Our initial means of studying this phenomenon is to understand tissue breakdown of collagen using SHG, and to monitor oxidative stress in the cell by following fluorescence of flavins and NADH.