FACTORS IMPORTANT FOR PATHOGENICITY IN CAMPYLOBACTER JEJUNI: BIOTERRORISM

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。空肠弯曲杆菌是一种重要的食源性致病菌，可引起一系列疾病，包括胃肠炎、直肠炎、败血症、流产、脑膜炎和上行性瘫痪。家禽、猪肉、牛肉或其他食物和水中只有800种微生物会导致疾病。每1000名接触者中就有1人会患上衰弱的神经系统疾病。空肠弯曲菌感染的分子机制目前知之甚少。家养动物是人类细菌的重要来源，但从动物转移到人类的机制尚不完全清楚。鸡、牛和猪携带空肠弯曲菌作为胃肠道共生菌，很少因这种细菌而发生临床疾病和病理。我们研究的长期目标是将弯曲杆菌从食物链中消灭。该项目的长期目标是开发蛋白质组学方法，作为密歇根州立大学研究界、兽医界、食用动物生产商、美国农业部、医学界和美国生物恐怖主义研究的资源。我们将与该中心的合作伙伴合作，建立使用自动化高通量二维电泳法对弯曲杆菌分离株进行分型的方法。我们已经收集了2500多个来自患有弯曲杆菌疾病的动物和人类的分离物，以及来自正常动物和环境的分离物。将为其中一些菌株建立蛋白质表达谱。一旦产生，将比较模式，探索独特的蛋白质的身份和功能，并将产生一个数据库。产生的信息将产生许多应用，但我们将专注于识别引起疾病的分离物共享的毒力属性，而不是非致病共生体中存在的毒力属性。从长远来看，我们将开发一种致病性空肠弯曲菌分离株的图谱，可以作为暴发和其他应用中分离株特征的快速筛查。这将作为一个示范项目，利用蛋白质组学开发病原微生物的自动化快速筛查。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Campylobacter jejuni is a significant emerging bacterial food borne pathogen of humans that causes a spectrum of diseases including gastroenteritis, proctitis, septicemia, abortion, meningitis, and ascending paralysis. As few as 800 organisms in poultry, pork, beef or other foods and water can cause disease. 1 out of 1000 exposed people develop debilitating neurological disease. Little is known about the molecular mechanisms involved in establishment of a C. jejuni infection. Domestic animals are a significant source of the bacterium for humans, but the mechanisms of transfer from animals to humans are not fully understood. Chickens, cattle and pigs carry C. jejuni as a GI commensal and only rarely develop clinical disease and pathology due to the bacterium. The long-term goal of our research is to eliminate Campylobacter from the food chain. The long-term goal of this project is to develop proteomic methods that could serve as a resource for the MSU research community, the veterinary community, food animal producers, USDA, the medical community, and US bioterrorism research. We will work with our collaborators in the Center to establish methods for typing Campylobacter isolates using automated high throughput 2 dimensional electrophoresis methods. We have assembled over 2500 isolates from animals and humans with disease due to Campylobacter, as well as isolates from normal animals and the environment. Protein expression profiles will be established for some of these strains. Once generated, patterns will be compared and unique proteins explored for identity and function, and a database will be generated. Numerous applications will arise from the information generated, but we will focus on identifying virulence attributes shared by isolates causing disease and not present in nonpathogenic commensals. In the long term we will develop a profile for pathogenic C. jejuni isolates that could serve as a rapid screen for characterizing isolates in outbreaks and other applications. This will serve as a demonstration project for developing automated rapid screens for pathogenic microorganisms using proteomics.