FACTORS IMPORTANT FOR PATHOGENICITY IN CAMPYLOBACTER JEJUNI: BIOTERRORISM

空肠弯曲菌致病性的重要因素：生物恐怖主义

• 批准号: 7602893
• 负责人: LINDA S. MANSFIELD
• 金额: $ 2.33万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602893
• 关键词: Animal Feed; Animals; Bacteria; Bioterrorism; Bioterrorism Research; Campylobacter; Campylobacter jejuni; Cattle; Chickens; Clinical; Communities; Computer Retrieval of Information on Scientific Projects Database; Databases; Disease; Disease Outbreaks; Domestic Animals; Domestic Fowls; Electrophoresis; Environment; Family suidae; Food; Food Chain; Funding; Gastroenteritis; Goals; Grant; Human; Infection; Institution; Medical; Meningitis; Methods; Molecular; Molecular Profiling; Organism; Paralysed; Pathogenicity; Pathology; Pattern; Proctitis; Proteins; Proteomics; Research; Research Personnel; Resources; Septicemia; Source; Sus scrofa; United States National Institutes of Health; Virulence; Water; Work; abortion; beef; foodborne pathogen; microorganism; nervous system disorder; protein expression; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Campylobacter jejuni is a significant emerging bacterial food borne pathogen of humans that causes a spectrum of diseases including gastroenteritis, proctitis, septicemia, abortion, meningitis, and ascending paralysis. As few as 800 organisms in poultry, pork, beef or other foods and water can cause disease. 1 out of 1000 exposed people develop debilitating neurological disease. Little is known about the molecular mechanisms involved in establishment of a C. jejuni infection. Domestic animals are a significant source of the bacterium for humans, but the mechanisms of transfer from animals to humans are not fully understood. Chickens, cattle and pigs carry C. jejuni as a GI commensal and only rarely develop clinical disease and pathology due to the bacterium. The long-term goal of our research is to eliminate Campylobacter from the food chain. The long-term goal of this project is to develop proteomic methods that could serve as a resource for the MSU research community, the veterinary community, food animal producers, USDA, the medical community, and US bioterrorism research. We will work with our collaborators in the Center to establish methods for typing Campylobacter isolates using automated high throughput 2 dimensional electrophoresis methods. We have assembled over 2500 isolates from animals and humans with disease due to Campylobacter, as well as isolates from normal animals and the environment. Protein expression profiles will be established for some of these strains. Once generated, patterns will be compared and unique proteins explored for identity and function, and a database will be generated. Numerous applications will arise from the information generated, but we will focus on identifying virulence attributes shared by isolates causing disease and not present in nonpathogenic commensals. In the long term we will develop a profile for pathogenic C. jejuni isolates that could serve as a rapid screen for characterizing isolates in outbreaks and other applications. This will serve as a demonstration project for developing automated rapid screens for pathogenic microorganisms using proteomics.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 空肠弯曲杆菌是人类的一种重要的新兴细菌性食物，可引​​起多种疾病，包括胃肠炎，肠炎，败血症，败血病，流产，脑膜炎和逐渐麻痹。 家禽，猪肉，牛肉或其他食物和水中只有800种生物会引起疾病。 1000人中，有1人患有使神经系统疾病衰弱的人。 关于建立空肠梭菌感染的分子机制知之甚少。 家畜是人类细菌的重要来源，但尚未完全了解从动物到人类转移的机制。鸡，牛和猪携带着空肠梭状芽孢杆菌作为胃肠道，仅由于细菌而很少出现临床疾病和病理。我们研究的长期目标是消除食物链中的弯曲杆菌。 该项目的长期目标是开发蛋白质组学方法，可以作为MSU研究界，兽医社区，食品动物生产者，USDA，医学界和美国生物恐怖主义研究的资源。 我们将与中心的合作者合作，以建立使用自动高吞吐量2尺寸电泳方法键入弯曲杆菌分离株的方法。 由于弯曲杆菌，我们已经从动物和人类中组装了2500多个分离株，以及来自正常动物和环境的分离株。这些菌株将建立蛋白质表达谱。一旦生成，将比较模式，并探讨了针对身份和功能的唯一蛋白质，并将生成数据库。 生成的信息将引起许多应用，但我们将集中精力确定由疾病造成疾病而不存在的疾病共享的毒力属性。 从长远来看，我们将开发一种用于病原梭菌分离株的曲线，该分离物可以作为在暴发和其他应用中表征分离株的快速筛选。 这将作为一个示范项目，用于开发使用蛋白质组学的致病微生物自动快速筛选。