Development of new tools and resources to enhance wheat functional genomics

点击翻译按钮获取中文摘要

We propose to improve and exploit recently developed technologies, which utilise defective mismatch repair genes to create a collection of wheat plants containing a high frequency of random mutations that can be screened either by direct sequence analysis or TILLING-based procedures. This collection will be a valuable resource for the UK wheat community, as it would represent a source of new and novel alleles for agronomically important genes. We propose to open up the resource to the entire wheat community as an unencumbered resource free of MTAs and follow on IP agreements. The procedure utilises transgenic technology to create new alleles, however, any novel or useful alleles identified can easily be separated from the transgene by conventional crossing and segregation.