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A multi-channel liquid handler for the Bristol Genomics facility

布里斯托尔基因组学设施的多通道液体处理器

基本信息

批准号：
BB/S019219/1
负责人：
Keith Edwards
金额：
$ 22.83万
依托单位：
University of Bristol
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2019
资助国家：
英国
起止时间：
2019 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FS019219%2F1
关键词：
multi channel liquid handler Bristol

项目摘要

Genomics technologies, specifically sequencing and genotyping, continue to witness exciting developments in both increased scale and reduced costs.The Bristol Genomics Facility collaborates closely with a number of groups at Bristol including the Cereals Functional Genomics group , who remain at the cutting edge of technology development; via collaborations with Affymetrix and LGC a number of commercially successful products have been developed and utilised such as the Axiom 820K wheat high density array and the Axiom 35K wheat breeders array. These developments have resulted in Bristol becoming a centre of high throughput genotyping for multiple commercially important species following the installation of a 384-well Axiom GeneTitan high throughput genotyping platform in 2014 (ALERT 13). In 2015 the University provided funds for the facility to purchase both an Illumina MiSeq and NextSeq500 next generation sequencer. These various developments have resulted in the Facility processing over 120,000 DNA samples over the past four years. To continue to be at the forefront of technology development and application the Facility must maintain and improve its post-PCR sampling handling provision and procedures so that it is able to cope with the ever increasing demand for its services. Provision of a high throughput liquid handling instrument would enable to Bristol Genomics Facility to both develop new strategies (such as targeted genotyping by sequencing) and expand the number of applications that it can offer its internal and external users.

基因组学技术，特别是测序和基因分型，继续见证了令人兴奋的发展，在增加规模和降低成本。布里斯托基因组学设施密切合作，与一些小组在布里斯托，包括谷物功能基因组学组，谁仍然在技术发展的前沿;通过与Affytechnology和LGC的合作，已经开发和利用了许多商业上成功的产品，例如Axiom 820 K小麦高密度阵列和Axiom 35 K小麦育种器阵列。这些发展使得布里斯托在2014年安装了384孔Axiom GeneTitan高通量基因分型平台（ALERT 13）后，成为多个商业重要物种的高通量基因分型中心。2015年，该大学为该设施提供资金，用于购买Illumina MiSeq和NextSeq 500下一代测序仪。由于这些不同的发展，该设施在过去四年中处理了120 000多份DNA样本。为了继续处于技术开发和应用的前沿，该设施必须维持和改进其聚合酶链式反应后取样处理规定和程序，以便能够科普对其服务日益增长的需求。提供高通量液体处理仪器将使布里斯托基因组学机构能够开发新的策略（例如通过测序进行靶向基因分型），并扩大其可以向内部和外部用户提供的应用数量。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2.

DOI：
10.1111/pbi.13573
发表时间：
2021-08
期刊：
Plant biotechnology journal
影响因子：
13.8
作者：
Raffan S;Sparks C;Huttly A;Hyde L;Martignago D;Mead A;Hanley SJ;Wilkinson PA;Barker G;Edwards KJ;Curtis TY;Usher S;Kosik O;Halford NG
通讯作者：
Halford NG

Crossover-active regions of the wheat genome are distinguished by DMC1, the chromosome axis, H3K27me3, and signatures of adaptation.

DOI：
10.1101/gr.273672.120
发表时间：
2021-09
期刊：
Genome research
影响因子：
7
作者：
Tock AJ;Holland DM;Jiang W;Osman K;Sanchez-Moran E;Higgins JD;Edwards KJ;Uauy C;Franklin FCH;Henderson IR
通讯作者：
Henderson IR