Purchase of Affymetrix GeneTitan for use in high throughput genotyping

购买 Affymetrix GeneTitan 用于高通量基因分型

• 批准号: BB/L014106/1
• 负责人: Keith Edwards
• 金额: $ 41.57万
• 依托单位: University of Bristol
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FL014106%2F1
• 关键词: Purchase; Affymetrix; GeneTitan; use; throughput

Funds are requested to purchase both a GeneTitan-Multi Channel (GeneTitan) genotyping system and a 5-year warranty. The application to purchase a GeneTitan has been instigated by Professor Keith Edwards, the academic director of the Bristol Genomics Facility, following discussions with several researchers and industrial partners both at Bristol and beyond. Researchers who would directly benefit from the placing of a GeneTitan at Bristol include Dr Eric Morgan's Bristol-based research into animal health and livestock production, Dr Amanda Churchill's Bristol-based research into ageing and wellbeing, Professor Ian King's Nottingham-based research into food security, Professor Andy Greenland's research into food security at NIAB, Professor Dr Julie Williams' Cardiff-based research into the disease processes associated with ageing and Dr Peter Jack's wheat breeding at RAGT to address issues associated with food security. All of these cases are in addition to Professor Keith Edwards' own extensive research into wheat breeding and its relationship to food security.The scientific cases for support all require high throughput/high density genotyping; the GeneTitan enables this on a scale required for genome wide genotyping applications. The GeneTitan achieves this by automating array processing from target hybridization to data generation by combining the required components (hybridization oven, fluidics processing, and state-of-the art imaging device) into a single bench-top instrument. As such the GeneTitan provides the highest throughput and laboratory productivity per operator by minimizing user intervention and allowing unattended, overnight processing of large numbers of samples in parallel. This degree of hands-free automation means that data is consistently reproducible, an essential attribute of gene expression studies in particular, and means that the time typically taken for hands-on array processing is available for data analysis increasing the time to meaningful results for research.

申请资金用于购买GeneTitan- multi Channel （GeneTitan）基因分型系统和5年质保。购买GeneTitan的申请是由布里斯托尔基因组研究所的学术主任基思·爱德华兹教授发起的，他与布里斯托尔和其他地方的几位研究人员和工业合作伙伴进行了讨论。直接受益于在布里斯托尔安置GeneTitan的研究人员包括埃里克·摩根博士在布里斯托尔对动物健康和牲畜生产的研究，阿曼达·丘吉尔博士在布里斯托尔对老龄化和福祉的研究，伊恩·金教授在诺丁汉对食品安全的研究，安迪·格陵兰教授在NIAB对食品安全的研究，朱莉·威廉姆斯教授在卡迪夫进行的与衰老相关的疾病过程研究，以及彼得·杰克博士在拉格特的小麦育种，以解决与粮食安全相关的问题。所有这些案例都是基思·爱德华兹教授本人对小麦育种及其与粮食安全关系的广泛研究的补充。支持的科学案例都需要高通量/高密度基因分型；GeneTitan能够在全基因组基因分型应用所需的规模上实现这一点。GeneTitan通过将所需组件（杂交烘箱、流体处理和最先进的成像设备）组合到单个台式仪器中，实现了从目标杂交到数据生成的自动化阵列处理，从而实现了这一点。因此，GeneTitan通过最大限度地减少用户干预和允许无人值勤、夜间并行处理大量样品，为每个操作人员提供最高的吞吐量和实验室生产率。这种程度的自动化意味着数据是可重复的，这是基因表达研究的一个基本属性，也意味着通常用于动手阵列处理的时间可用于数据分析，从而增加了获得有意义的研究结果的时间。

项目成果

CerealsDB 3.0: expansion of resources and data integration.

CEATEALSDB 3.0：资源和数据集成的扩展。

• DOI: 10.1186/s12859-016-1139-x
• 发表时间: 2016-06-24
• 期刊: BMC bioinformatics
• 影响因子: 3
• 作者: Wilkinson PA;Winfield MO;Barker GL;Tyrrell S;Bian X;Allen AM;Burridge A;Coghill JA;Waterfall C;Caccamo M;Davey RP;Edwards KJ
• 通讯作者: Edwards KJ

Characterization of polyploid wheat genomic diversity using a high-density 90,000 single nucleotide polymorphism array.

• DOI: 10.1111/pbi.12183
• 发表时间: 2014-08
• 期刊: Plant biotechnology journal
• 影响因子: 13.8
• 作者: Wang S;Wong D;Forrest K;Allen A;Chao S;Huang BE;Maccaferri M;Salvi S;Milner SG;Cattivelli L;Mastrangelo AM;Whan A;Stephen S;Barker G;Wieseke R;Plieske J;International Wheat Genome Sequencing Consortium;Lillemo M;Mather D;Appels R;Dolferus R;Brown-Guedira G;Korol A;Akhunova AR;Feuillet C;Salse J;Morgante M;Pozniak C;Luo MC;Dvorak J;Morell M;Dubcovsky J;Ganal M;Tuberosa R;Lawley C;Mikoulitch I;Cavanagh C;Edwards KJ;Hayden M;Akhunov E
• 通讯作者: Akhunov E

A step change in the transfer of interspecific variation into wheat from Amblyopyrum muticum.

种间变异转移到小麦中的步骤变化。

• DOI: 10.1111/pbi.12606
• 发表时间: 2017-02
• 期刊: Plant biotechnology journal
• 影响因子: 13.8
• 作者: King J;Grewal S;Yang CY;Hubbart S;Scholefield D;Ashling S;Edwards KJ;Allen AM;Burridge A;Bloor C;Davassi A;da Silva GJ;Chalmers K;King IP
• 通讯作者: King IP

Conversion of array-based single nucleotide polymorphic markers for use in targeted genotyping by sequencing in hexaploid wheat (Triticum aestivum).

• DOI: 10.1111/pbi.12834
• 发表时间: 2018-04
• 期刊: Plant biotechnology journal
• 影响因子: 13.8
• 作者: Burridge AJ;Wilkinson PA;Winfield MO;Barker GLA;Allen AM;Coghill JA;Waterfall C;Edwards KJ
• 通讯作者: Edwards KJ

Measuring stress signaling responses of stomata in isolated epidermis of graminaceous species.

• DOI: 10.3389/fpls.2015.00533
• 发表时间: 2015
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Shen L;Sun P;Bonnell VC;Edwards KJ;Hetherington AM;McAinsh MR;Roberts MR
• 通讯作者: Roberts MR