High throughput sample handling for high throughput genomics

资金要求购买一个高通量样品处理套件连同5年保修。购买该套件的应用程序已煽动教授基思爱德华兹，布里斯托基因组学设施的学术主任，讨论后与几个研究人员和工业合作伙伴都在布里斯托和超越。将直接受益于在布里斯托安装套件的研究人员包括阿曼达博士丘吉尔的布里斯托尔为基础的研究老龄化和福祉，伊恩金教授的诺丁汉为基础的研究粮食安全，艾莉森宾利博士的研究粮食安全在NIAB和克里斯伯特博士的小麦育种在RAGT解决与粮食安全相关的问题。所有这些案例都是除了基思爱德华兹教授自己对小麦育种及其与粮食安全的关系的广泛研究以及加里巴克博士在宏基因组学方面的研究之外。支持这些科学案例的所有科学案例都需要高通量测序和基因分型;高通量样品处理套件能够在基因组范围应用所需的规模上实现这一点，例如通过测序和基因组扫描进行基因分型。高通量处理套件由两个主要组件组成; 1. Biomek 4000是一款液体处理工作站，支持多种应用，包括DNA提取、扩增、设置、样品稀释和标准化。Covaris E220是一种聚焦超声波仪，能够进行多样品、批量制备，并处理各种样品类型和体积。E220可编程为一批处理多达96个样品，每个样品具有不同的超声处理要求和体积。这使得有可能将优点带给许多生物和化学应用，包括DNA，其是用于下一代测序的基因组DNA文库制备方法的标准部分。虽然还有其他可用的自动化平台，但我们要求提供最大灵活性的平台，以支持现有管道并支持新应用程序的开发，而不是限制投资使用的封闭系统机器人

Funds are requested to purchase a High Throughput Sample Handling Suite together with a 5-year warranty.The application to purchase the suite has been instigated by Professor Keith Edwards, the academic director of the Bristol Genomics Facility, following discussions with several researchers and industrial partners both at Bristol and beyond. Researchers who would directly benefit from installing the Suite at Bristol include Dr Amanda Churchill's Bristol-based research into ageing and wellbeing, Professor Ian King's Nottingham-based research into food security, Dr Alison Bentley's research into food security at NIAB and Dr Chris Burt's wheat breeding at RAGT to address issues associated with food security. All of these cases are in addition to Professor Keith Edwards' own extensive research into wheat breeding and its relationship to food security and Dr Gary Barker's research in metagenomicsThe scientific cases for support all require high throughput sequencing and genotyping; the high throughput sample handling suite enables this on a scale required for genome wide applications such as genotyping by sequencing and genome scanning. The high throughput handling suite consists of two main components; 1. The Biomek 4000 is a liquid handling workstation supporting a variety of applications including DNA extraction, amplification, setup, sample dilution and normalization, 2. The Covaris E220 is a focussed ultra-sonicator capable of multi-sample, batch preparation, and processing a wide range of sample types and volumes. The E220 may be programed to process up to 96 samples in a single batch each with variable sonication requirements and volumes. This makes it possible to bring the advantages to numerous biological and chemical applications including, DNA which is a standard part of genomic DNA library preparation methods for next generation sequencing. Whilst there are other automation platforms available, we are requesting one that provides maximum flexibility to feed into existing pipelines and support the development of new applications, as opposed to closed system robotics which would limit the usage of the investment