INTERACTIONS IN PCOS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。具体目的是验证这样一种假设，即17b-HSD5基因的激活变体的表型取决于与LH、胰岛素抵抗性高胰岛素血症和FSH依赖性颗粒细胞因子的相互作用。我们将首先确定PCOS和已知17b-HSD5基因型对照女性中睾酮对LH类似物人慢性促性腺激素（hCG）的反应。然后，我们将确定用GnRH激动剂抑制促性腺激素的作用，用噻唑烷二酮（TZD）抑制胰岛素，用FSH刺激颗粒细胞功能。还将评估用TZD抑制胰岛素对LH脉冲特征和胰岛素分泌/敏感性指标的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aim is to test the hypothesis that the phenotype of an activating variant of the 17b-HSD5 gene depends on interactions with LH, insulin resistant hyperinsulinemia, and FSH dependent granulosa cell factors. We will begin by determining the testosterone response to the LH analog human chronic gonadotropin (hCG) in PCOS and control women of known 17b-HSD5 genotype. Then we will determine the effects of suppressing gonadotropins with a GnRH agonist, suppressing insulin with a thiazolidinedione (TZD), and stimulating granulosa cell function with FSH. The effects of suppressing insulin with a TZD on LH pulse characteristics and indexes of insulin secretion/sensitivity will also be assessed.