A laser cutting and laser capture microscope for plant biology

多细胞生物拥有包含多种细胞类型的组织。每一种细胞类型都不同，以执行定义的功能。我们感兴趣的是发生在特定细胞中的过程。目的是确定在特定细胞中允许差异基因表达的机制，以及这些细胞中这种差异表达的后果。例如，申请人1的目标是确定细胞类型之间启动子元件甲基化的差异，申请人3-5希望确定特定细胞类型的转录物丰度，然后使用该信息生成发育和昼夜节律网络，或了解对病原体攻击或细胞信号传导的反应。在分析基因表达之前，通过LCM收集的植物细胞只有10个，因此所有提出的工作都是可行的，我们应该在我们的每个领域取得重大进展。

Mutlicellular organisms possess tissues containing various cell types. Each cell type differentiates to perform defined functions. We are interested in processes that occur in specific cells. The aim is to identify mechanisms allowing differential gene expression in particular cells, and the consequences of this differential expression in those cells. For example, Applicant 1 aims to identify differences in methylation of promoter elements between cell-types, applicants 3-5 wish to determine transcript abundance from specific cell-types, and then use that information to generate developmental and circadian networks, or understand responses to pathogen attack or cell signalling. As few as ten plant cells have been collected via LCM prior to analysis of gene expression, and so all the work proposed is feasible and we should make significant advances in each of our fields.