COBRE: RIH: CORE C: MOLECULAR PATHOLOGY, TRANSGENIC RAT, ANIMAL MODEL OF CANCER

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。该中心为病理科、COBRE中心和学术界的研究工作提供了最先进的仪器和支持人员。收费服务包括激光捕获显微解剖，定量实时PCR，组织微阵列生产，肿瘤银行，自动免疫组织化学染色，数据和图像分析。1300平方。该设施位于罗德岛医院的奥尔德里奇大楼。迄今为止，共有32个研究小组使用了我们的服务和设施。组织库：寿命/罗德岛医院和米里亚姆医院说明：为研究目的提供新鲜冷冻的人类肿瘤和正常组织样本。这些组织样本代表了在罗德岛医院和米里亚姆医院手术切除的标本的横截面（包括：结肠、胃、肺、乳房、甲状腺、肾脏、胰腺和各种其他器官）。通常配对的肿瘤和正常组织样本是从每个可用标本中提取的。样品在液氮中快速冷冻并在80℃下储存，有关每个样品的病理诊断的详细信息也可根据要求提供。所有样品申请都由一个由三名医生组成的委员会进行评估，批准严格基于研究的优点。根据HIPAA和机构审查委员会（IRB）的规定，Lifespan Tissue Bank是一家匿名的非营利性银行。可用性：调查人员需要填写详细的组织申请表，可通过我们的网站：www.rih-cobre-cares.org获得。需要IRB项目批准。联系：Dilip Giri， M.D, 401-444-3122，或DGiri@Lifespan.org BioAnalyzer QC。我们使用安捷伦生物分析仪运行了106个纳米芯片和Picochips（每个芯片分别进行12和11次测试），以支持从单个细胞中提取RNA用于QPCR和DNA微阵列的实验。主要设备Arturus Autopix激光捕获显微解剖系统Ventana发现系统Agilent生物分析仪Stratagene多重PCR系统显微镜成像系统Leica Cryostat组织微阵列仪Revco冷冻机(2)显微组新方向*激光捕获细胞的基因表达阵列：我们增加了DNA阵列分析石蜡包埋组织中捕获细胞的基因表达的能力。使用来自Arcturus的新Paradise系统，我们从FFPE激光捕获的细胞中分离、纯化和扩增RNA，用于基因芯片分析。*用于DNA分析的生物分析仪：我们增加了通过生物分析仪评估DNA的能力。*蛋白质组学激光从石蜡包埋组织中捕获细胞。我们对FFPE组织的细胞进行了显微解剖，并使用Expression Pathology的新型Liquid tisetm MS Protein Prep试剂盒，在我们的合作伙伴proteomics Core实验室提取足够数量和质量的肽，用于GC-MS蛋白质组学。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core provides state-of-the-art instrumentation and support personnel for the research efforts of the Department of Pathology, the COBRE Center, and academic community. Fee-based services include Laser Capture Microdissection, Quantitative Real-Time PCR, Tissue Microarray production, tumor banking, automated immunohistochemical staining, data and image analysis. Our 1300 sq. ft. facility is located in the Aldrich Building at Rhode Island Hospital. To date, a total of 32 research groups have made use of our services and facilities. TISSUE BANK: LIFESPAN/RHODE ISLAND HOSPITAL AND THE MIRIAM HOSPITAL Description: Provides fresh frozen human tumor and normal tissue sample for research purposes. The tissue samples represent a cross section of specimens (these include: colon, stomach, lung, breast, thyroid, kidney, pancreas and a variety of other organs) surgically resected at Rhode Island Hospital and The Miriam Hospital. Typically paired tumor and normal tissue samples are banked from each of the available specimens. Samples are snap frozen in liquid nitrogen and stored at ¿80degrees C. Details about the pathologic diagnosis for each sample are also provided upon request. All requests for samples are evaluated by a committee of three physicians, and approvals are based strictly on the merits of the research. The Lifespan Tissue Bank is an anonymous non-profit bank in accordance with HIPAA and Institutional Review Board (IRB) regulations. Availability: Investigators are required to complete a detailed tissue request form obtainable through our website at: www.rih-cobre-cares.org. IRB project approval is required. Cost: Refer to website for fee list: www.rih-cobre-cares.org Contact: Dilip Giri, M.D., 401-444-3122, or at DGiri@Lifespan.org BioAnalyzer QC We have run 106 Nanochips and Picochips (12 and 11 tests per chip, respectively) by Agilent Bioanalyzer in support of experiments to provide RNA from individual cells for QPCR and DNA microarrays. Major Equipment Arturus Autopix Laser Capture Microdissection System Ventana Discovery System Agilent Bioanalyzer Stratagene Multiplex PCR System Microscope Imaging System Leica Cryostat Tissue Microarrayer Revco Freezers (2) Microtome New Directions * Gene Expression Arrays from laser captured cells: We have added the capability for DNA array analysis of gene expression in cells captured from paraffin embedded tissues. Using the new Paradise system from Arcturus we have isolated, purified and amplified, RNA from FFPE laser captured cells for gene chip analysis. * BioAnalyser for DNA analysis: We have added the ability to evaluate DNA by BioAnalyser. * Proteomics laser captured cells from paraffin embedded tissues. We have microdissected cells from FFPE tissue and used the new Liquid TissueTM MS Protein Prep kit from Expression Pathology to extract peptides of sufficient quantity and quality for GC-MS proteomics at our companion Proteomics Core laboratory.