COBRE: RIH: CORE C: MOLECULAR PATHOLOGY, TRANSGENIC RAT, ANIMAL MODEL OF CANCER

COBRE：RIH：核心 C：分子病理学、转基因大鼠、癌症动物模型

• 批准号: 7381872
• 负责人: RONALD DELELLIS
• 金额: $ 17.17万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381872
• 关键词: COBRE; RIH; CORE; MOLECULAR; PATHOLOGY

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core provides state-of-the-art instrumentation and support personnel for the research efforts of the Department of Pathology, the COBRE Center, and academic community. Fee-based services include Laser Capture Microdissection, Quantitative Real-Time PCR, Tissue Microarray production, tumor banking, automated immunohistochemical staining, data and image analysis. Our 1300 sq. ft. facility is located in the Aldrich Building at Rhode Island Hospital. To date, a total of 32 research groups have made use of our services and facilities. TISSUE BANK: LIFESPAN/RHODE ISLAND HOSPITAL AND THE MIRIAM HOSPITAL Description: Provides fresh frozen human tumor and normal tissue sample for research purposes. The tissue samples represent a cross section of specimens (these include: colon, stomach, lung, breast, thyroid, kidney, pancreas and a variety of other organs) surgically resected at Rhode Island Hospital and The Miriam Hospital. Typically paired tumor and normal tissue samples are banked from each of the available specimens. Samples are snap frozen in liquid nitrogen and stored at ¿80degrees C. Details about the pathologic diagnosis for each sample are also provided upon request. All requests for samples are evaluated by a committee of three physicians, and approvals are based strictly on the merits of the research. The Lifespan Tissue Bank is an anonymous non-profit bank in accordance with HIPAA and Institutional Review Board (IRB) regulations. Availability: Investigators are required to complete a detailed tissue request form obtainable through our website at: www.rih-cobre-cares.org. IRB project approval is required. Cost: Refer to website for fee list: www.rih-cobre-cares.org Contact: Dilip Giri, M.D., 401-444-3122, or at [e-mail] BioAnalyzer QC We have run 106 Nanochips and Picochips (12 and 11 tests per chip, respectively) by Agilent Bioanalyzer in support of experiments to provide RNA from individual cells for QPCR and DNA microarrays. Major Equipment Arturus Autopix Laser Capture Microdissection System Ventana Discovery System Agilent Bioanalyzer Stratagene Multiplex PCR System Microscope Imaging System Leica Cryostat Tissue Microarrayer Revco Freezers (2) Microtome New Directions * Gene Expression Arrays from laser captured cells: We have added the capability for DNA array analysis of gene expression in cells captured from paraffin embedded tissues. Using the new Paradise system from Arcturus we have isolated, purified and amplified, RNA from FFPE laser captured cells for gene chip analysis. * BioAnalyser for DNA analysis: We have added the ability to evaluate DNA by BioAnalyser. * Proteomics laser captured cells from paraffin embedded tissues. We have microdissected cells from FFPE tissue and used the new Liquid TissueTM MS Protein Prep kit from Expression Pathology to extract peptides of sufficient quantity and quality for GC-MS proteomics at our companion Proteomics Core laboratory.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。该核心为病理学系，COBRE中心和学术界的研究工作提供了最先进的仪器和支持人员。收费服务包括激光捕获显微切割、定量实时PCR、组织微阵列制作、肿瘤库、自动免疫组织化学染色、数据和图像分析。我们的1300平方米英尺机构位于罗得岛医院的奥尔德里奇大楼。迄今为止，共有32个研究小组使用了我们的服务和设施。 组织库：LIFESPAN/罗得岛医院和米里亚姆医院描述：提供新鲜冷冻的人类肿瘤和正常组织样本用于研究目的。组织样本代表在罗得岛医院和Miriam医院手术切除的标本（包括：结肠、胃、肺、乳腺、甲状腺、肾脏、胰腺和各种其他器官）的横截面。通常，从每个可用标本中储存成对的肿瘤和正常组织样品。将样品在液氮中快速冷冻并储存在± 80 ℃下。关于每个样本的病理诊断的详细信息也可根据要求提供。所有的样本请求都由一个由三名医生组成的委员会进行评估，批准严格基于研究的优点。 Lifespan Tissue Bank是一家符合HIPAA和机构审查委员会（IRB）规定的匿名非营利银行。 供货情况：要求研究者填写一份详细的组织申请表，可通过我们的网站www.rih-cobre-cares.org获得。需要IRB项目批准。费用：请参阅网站费用清单：www.rih-cobre-cares.org联系人：Dilip Giri，医学博士，401-444-3122，或在[e-mail] BioAnalyzer QC我们已经通过Agilent Bioanalyzer运行了106个纳米芯片和微微芯片（每个芯片分别进行12次和11次测试），以支持实验，从而为QPCR和DNA微阵列提供来自单个细胞的RNA。 主要设备Arturus Autopix激光捕获显微切割系统Ventana Discovery系统Agilent生物分析仪Stratagene多重PCR系统显微镜成像系统Leica Cryostat组织微阵列仪Revco冷冻机（2）切片机New Directions * 激光捕获细胞的基因表达阵列：我们增加了DNA阵列分析能力，可以分析从石蜡包埋组织捕获的细胞中的基因表达。利用大角星的新天堂系统，我们从FFPE激光捕获的细胞中分离、纯化和扩增RNA用于基因芯片分析。* 用于DNA分析的BioAnalyser：我们增加了通过BioAnalyser评估DNA的功能。* 蛋白质组学激光从石蜡包埋的组织中捕获细胞。我们从FFPE组织中显微切割细胞，并使用Expression Pathology的新型Liquid TissueTM MS Protein Prep试剂盒，在我们的配套蛋白质组学核心实验室中提取足够数量和质量的肽用于GC-MS蛋白质组学。