COBRE: RIH: CORE C: MOLECULAR PATHOLOGY, TRANSGENIC RAT, ANIMAL MODEL OF CANCER

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。该中心为病理学系、科布雷中心和学术界的研究工作提供最先进的仪器和支持人员。收费服务包括激光捕获显微解剖、定量实时聚合酶链式反应、组织微阵列生产、肿瘤库、自动免疫组织化学染色、数据和图像分析。我们的1300平方英尺。英国《金融时报》设施位于罗德岛医院的奥尔德里奇大楼。到目前为止，共有32个研究小组使用了我们的服务和设施。组织银行：LIFESPAN/罗德岛医院和米里亚姆医院描述：提供新鲜的冷冻人体肿瘤和正常组织样本用于研究目的。这些组织样本代表了罗德岛医院和米里亚姆医院手术切除的标本的横截面(包括：结肠、胃、肺、乳房、甲状腺、肾脏、胰腺和各种其他器官)。通常，配对的肿瘤和正常组织样本是从每个可用的样本中储存的。样品在液氮中快速冷冻，并储存在摄氏？80度。还可根据要求提供每个样品的病理诊断细节。所有索取样本的请求都由一个由三名医生组成的委员会进行评估，批准严格基于研究的价值。根据HIPAA和机构审查委员会(IRB)的规定，LIFESPAN组织银行是一家匿名的非营利银行。可获得性：调查人员需要填写一份详细的组织申请表，可通过我们的网站www.rih-cobre-cares.org获得。需要IRB项目批准。费用：请访问网站查看费用清单：www.rih-cobre-cares.org联系方式：Dilip Giri，M.D.，401-444-3122，或发送电子邮件至dGiri@Lifespan.org生物分析器QC我们已经运行了安捷伦生物分析器的106个纳米芯片和微微芯片(每个芯片分别进行12次和11次测试)，以支持为QPCR和DNA微阵列提供单个细胞RNA的实验。主要设备Arturus Autopix激光捕获显微切割系统Ventana Discovery系统安捷伦生物分析仪Stratagene多重聚合酶链式反应系统显微镜成像系统徕卡冷冻组织微阵列Revco冷冻机(2)激光捕获细胞的基因表达阵列：我们增加了对从石蜡包埋组织捕获的细胞中的基因表达进行DNA阵列分析的能力。利用Arcturus新的Paradise系统，我们从FFPE激光捕获细胞中分离、纯化和扩增了RNA，用于基因芯片分析。*用于DNA分析的BioAnalyser：我们增加了使用BioAnalyser评估DNA的能力。*蛋白质组学激光捕获石蜡包埋组织中的细胞。我们已经从FFPE组织中显微解剖了细胞，并在我们的配套蛋白质组学核心实验室使用了来自表达病理学的新型Liquid TIssueTM MS Protein Prep Kit提取了足够数量和质量的多肽，用于GC-MS蛋白质组学。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core provides state-of-the-art instrumentation and support personnel for the research efforts of the Department of Pathology, the COBRE Center, and academic community. Fee-based services include Laser Capture Microdissection, Quantitative Real-Time PCR, Tissue Microarray production, tumor banking, automated immunohistochemical staining, data and image analysis. Our 1300 sq. ft. facility is located in the Aldrich Building at Rhode Island Hospital. To date, a total of 32 research groups have made use of our services and facilities. TISSUE BANK: LIFESPAN/RHODE ISLAND HOSPITAL AND THE MIRIAM HOSPITAL Description: Provides fresh frozen human tumor and normal tissue sample for research purposes. The tissue samples represent a cross section of specimens (these include: colon, stomach, lung, breast, thyroid, kidney, pancreas and a variety of other organs) surgically resected at Rhode Island Hospital and The Miriam Hospital. Typically paired tumor and normal tissue samples are banked from each of the available specimens. Samples are snap frozen in liquid nitrogen and stored at ¿80degrees C. Details about the pathologic diagnosis for each sample are also provided upon request. All requests for samples are evaluated by a committee of three physicians, and approvals are based strictly on the merits of the research. The Lifespan Tissue Bank is an anonymous non-profit bank in accordance with HIPAA and Institutional Review Board (IRB) regulations. Availability: Investigators are required to complete a detailed tissue request form obtainable through our website at: www.rih-cobre-cares.org. IRB project approval is required. Cost: Refer to website for fee list: www.rih-cobre-cares.org Contact: Dilip Giri, M.D., 401-444-3122, or at DGiri@Lifespan.org BioAnalyzer QC We have run 106 Nanochips and Picochips (12 and 11 tests per chip, respectively) by Agilent Bioanalyzer in support of experiments to provide RNA from individual cells for QPCR and DNA microarrays. Major Equipment Arturus Autopix Laser Capture Microdissection System Ventana Discovery System Agilent Bioanalyzer Stratagene Multiplex PCR System Microscope Imaging System Leica Cryostat Tissue Microarrayer Revco Freezers (2) Microtome New Directions * Gene Expression Arrays from laser captured cells: We have added the capability for DNA array analysis of gene expression in cells captured from paraffin embedded tissues. Using the new Paradise system from Arcturus we have isolated, purified and amplified, RNA from FFPE laser captured cells for gene chip analysis. * BioAnalyser for DNA analysis: We have added the ability to evaluate DNA by BioAnalyser. * Proteomics laser captured cells from paraffin embedded tissues. We have microdissected cells from FFPE tissue and used the new Liquid TissueTM MS Protein Prep kit from Expression Pathology to extract peptides of sufficient quantity and quality for GC-MS proteomics at our companion Proteomics Core laboratory.