DETERMINING AQUATIC BIOINDICATORS FOR ATRAZINE

测定阿特拉津的水生生物指示剂

• 批准号: 7381577
• 负责人: ANDRE W DELORME
• 金额: $ 18.86万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381577
• 关键词: DETERMINING; AQUATIC; BIOINDICATORS; ATRAZINE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The occurrence specific macroinvertebrates in the Sheyenne and Wild Rice rivers will be correlated with Atrazine levels. An HPLC protocol is being developed for the quantitation of Atrazine and its metabolites to parts per billion (ppb). Enzyme-Linked Immunosorbent Assay (ELISA) kits have recently been approved by the EPA?s Environmental Technology Verification (ETV) program for use in detecting Atrazine in the ppb level. We intend to use the kits as a screen, follwed by precise determination via HPLC. Our second specific aim is to identify the macroinvertebrates in the two river systems and correlate their occurrence with Atrazine levels. We carried out macroinvertebrate sampling on six sites on the Wild Rice in late August, collecting approximately 300 individuals from the commonly occuring family Chironomidae, (midges). All individuals were identified to at least the genus level using light microscopy. Based on previous studies on the Sheyenne River we have tentatively identified several taxa for future mesocosm experiments. These include the Chironomids Polypedilum, Cryptochironomus, Chironomus, and Procladius; the mayflies Hexagenia limbata and Caenis; the caddisfly Hydropsyche; the beetle Dubiraphia; and the amphipod Hyallela azteca. We have obtained GIS mapping of Atrazine usage in the Sheyenne River basin over the last several years and are waiting for maps of its usage in the Wild Rice basin. This year we will carry out a rigorous sampling regime for Atrazine in our two river basins. In addition we will continue to sample and monitor the macroinvertbrate fauna in those rivers and try and identify correlations with Atrazine levels. A major undertaking in the second year will be the purchase and set up of the first set of mescosms, or artificial streams. We hope to begin preliminary studies for our third specific aim, to examine any possible endocrine effects on macroinvertebrates raised in the mesocosms.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。 Sheyenne 和 Wild Rice 河流中出现的特定大型无脊椎动物将与莠去津水平相关。正在开发 HPLC 方案，用于将莠去津及其代谢物定量至十亿分之一 (ppb)。酶联免疫吸附测定 (ELISA) 试剂盒最近已获得 EPA 环境技术验证 (ETV) 计划的批准，可用于检测 ppb 水平的莠去津。我们打算使用该试剂盒作为筛选，然后通过 HPLC 进行精确测定。 我们的第二个具体目标是识别两个河流系统中的大型无脊椎动物，并将它们的出现与莠去津水平相关联。 8 月下旬，我们在野生稻的 6 个地点进行了大型无脊椎动物采样，从常见的摇蚊科（摇蚊科）中采集了约 300 只个体。使用光学显微镜将所有个体至少鉴定到属水平。根据之前对谢延河的研究，我们初步确定了未来中生态实验的几个分类群。其中包括多足摇蚊、隐摇蚊、摇蚊和普罗克拉迪斯；蜉蝣 Hexagenia Limbata 和 Caenis；石蛾 Hydropsyche；甲虫 Dubiraphia；和端足类Hyallela azteca。过去几年，我们已经获得了 Sheyenne 河流域阿特拉津使用情况的 GIS 地图，并且正在等待野生稻流域的阿特拉津使用情况地图。 今年，我们将对两个流域的莠去津进行严格的采样制度。此外，我们将继续对这些河流中的大型无脊椎动物群进行采样和监测，并尝试确定与莠去津水平的相关性。第二年的一项主要任务将是购买和建立第一套生态系统，即人工溪流。我们希望为我们的第三个具体目标开始初步研究，即检查对中生态系统中大型无脊椎动物的任何可能的内分泌影响。