MS INBRE UMMC: GENOMICS FACILITY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。UMC的基因组学设施是一个培训设施，提供访问通常但不完全与微阵列技术相关的专门设备的机会。基因组学设施正在稳步使用。使用中最活跃的设备是安捷伦生物分析仪(用于DNA和RNA分析)、扫描阵列快速扫描仪(微阵列分析)以及相邻的分析计算机和程序。整个医疗中心的许多实验室都定期使用该设施。该设施还扫描该州其他不在地铁杰克逊地区的实验室的幻灯片。目前，我们正在为密西西比州立大学的拉里·汉森·S博士实验室扫描鲶鱼微阵列。我们继续引进和培训新的微阵列技术实验室。尽管过去一年有几个设施用户申请了赠款，但没有人收到。然而，我们继续与其他几个实验室进行可行性研究，我们希望这些研究可以成为可资助的提案。一项研究酒精对斑马鱼表观遗传影响的提案将在目前的资助期内提交。我们还同意对多伦多大学健康科学网络和W.L.戈尔及其同事制造的一些微阵列进行Beta测试。此外，我们继续向病理住院医生演示RNA是如何标记、杂交和分析的。预防医学部的生物统计学家沃伦·梅博士与大多数通过该设施进行微阵列分析的小组合作。我们要求所有想要使用该设施的小组在开始之前就他们的实验设计与他进行磋商。还鼓励用户与南密西西比大学生物信息学专家、MFGN生物信息学分会联席主任邓友平博士进行咨询和合作。该设施的目标是通过向密西西比州内的所有学生和教师研究人员提供培训，帮助提高密西西比州研究和生命科学培训的质量和数量；培训他们在设施内进行研究的所有设备的方法和使用。工厂由厂长和工厂经理监督，几乎所有用户都可以随时使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Genomics Facility at UMC is a training facility that provides access to specialized equipment typically, but not entirely, associated with microarray technology. The genomics facility is seeing steady usage. The most active pieces of equipment being used are the Agilent bioanalyzer (for DNA and RNA analyses), the ScanArray Express scanner (microarray analysis) and the adjoining analytical computer and programs. Numerous labs throughout the Medical Center avail themselves of the facility on a regular basis. The facility also scans slides for other labs within the state that are not in the metro Jackson area. Presently, we are scanning catfish microarrays for Dr. Larry Hansen?s lab at Mississippi State University. We continue to introduce and train new labs in microarray technology. Although several facility users applied for grants this past year none were received. However we continue to do feasibility studies with several other labs which we hope can be garnered into fundable proposals. A proposal to study epigenetic effects of alcohol on zebrafish will be submitted within the current funding period. We have also agreed to beta-test some microarrays made by the University of Toronto Health Science network and W.L. Gore and Associates. Additionally we continue to demonstrate to pathology residents how RNA is labeled, hybridized and analyzed. Dr. Warren May, a Biostatistician in the Department of Preventive Medicine works with most of the groups doing microarray analysis through the facility. We require all groups wanting to use the facility to consult with him about the design of their experiments before beginning. Users are also encouraged to consult and collaborate with Dr. Youping Deng who is the bioinformatics expert at the University of Southern Mississippi and co-director of the bioinformatics branch of the MFGN. It is the goal of this facility to help facilitate the enhancement of the quality and quantity of research and life science training within the state of Mississippi through being available to all student and faculty researchers within the state; to train them in the methods and use of all of the equipment within the facility for their research. The facility is supervised by the director and facility manager and is available to all users at virtually anytime.