LSU DENTAL COBRE: ORAL HUMAN PAPILLOMAVIRUS INFECTION IN HIV-POSITIVE PATIENTS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。导语：通过高活性抗逆转录病毒治疗（HAART）， hiv阳性患者的全身免疫重建已经完成，许多口腔感染也随之减少。然而，最近的报告指出，口腔病理的增加与人类乳头瘤病毒（HPV）感染一致，这是一种在艾滋病毒阳性患者的口腔和生殖道中发现的病毒。该项目的目的是评估HAART对hiv阳性个体口腔HPV感染的影响。为此，将使用纵向收集的口腔拭子、唾液和血液以及病变阳性组织来检查艾滋病毒阳性个体对HPV的局部和全身免疫反应。目的：招募受试者参加本临床研究；建立实验室，配备必要的样品分析设备；成为一名科学研究者。方法：与指导团队合作，明确患者入组和分层，建立实验室；参加COBRE的教育活动。结果：以下是第一年的进展情况：受试者入组，获得口腔拭子，唾液和血液采集。从拭子和唾液细胞颗粒中提取DNA以检测HPV的存在，并将唾液上清冷冻以用于未来的细胞因子和HPV抗体检测。已聘用一名临床协调员（0.5 FTE），一名研究助理（0.5 FTE）和一名学生工作者。项目负责人正在与研究助理一起优化该项目所需的实验室技术。PI参加了COBRE赞助的月刊俱乐部、寻求资助者会议、综合临床培训课程、进度报告和演讲，并参加了NIH资助人讲习班、巴尔的摩2005年IADR/AADR年度会议和温哥华第22届国际乳头瘤病毒会议。结论：在指导团队的支持下，PI有望成为一名有能力获得独立资助的临床研究人员。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: Systemic immune reconstitution for the HIV-positive patient has been accomplished with highly active anti-retroviral therapy (HAART) with an associated decrease in many oral infections. However recent reports have noted increases in oral pathology consistent with human papillomavirus (HPV) infection, a virus found in both oral and genital tracts of HIV-positive patients. The goal of this project is to evaluate the impact of HAART on HPV infection in the oral cavity of HIV-positive individuals. To achieve this, longitudinally collected oral swabs, saliva and blood as well as lesion-positive tissue, will be used to examine local and systemic immune responses to HPV in HIV-positive individuals. Objective: To enroll subjects into this clinical study; to set-up a lab with the necessary equipment for sample analysis; to become educated as a scientific investigator. Methods: Working with the mentoring team to clarify patient enrollment and stratification and set up the lab; attend COBRE educational activities. Results: The following is progress from year 1: Subjects have been enrolled, oral swabs obtained, saliva and blood collected. DNA has been extracted from the swabs and salivary cell pellet to test for the presence of HPV and the saliva supernatant frozen for future cytokine and HPV antibody detection. A clinical coordinator 0.5 FTE, a research associate (0.5 FTE) and a student worker have been hired. The PI is working with the research associate to optimize the laboratory techniques necessary for this project. The PI participated in COBRE sponsored monthly journal club, grant seeker sessions, comprehensive clinical training course, progress reports and presentations as well as attending an NIH grantwriters workshop, the 2005 IADR/AADR annual meeting in Baltimore and the 22nd International Papillomavirus Conference in Vancouver. Conclusion: The PI with support from the mentoring team is poised to become a clinical researcher capable to obtaining independent funding.