COBRE: UND: MASS SPECTROMETRY CORE FACILITY

COBRE：UND：质谱核心设施

• 批准号: 7381904
• 负责人: MASARU MIYAGI
• 金额: $ 26.2万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381904
• 关键词: COBRE; UND; MASS; SPECTROMETRY; CORE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core facility supports the research of the COBRE investigators. Specifically, high-resolution electrospray-quadrupole/time-of-flight mass spectrometer is being used in Project 2 (Porter) for identification of pro-inflammatory arachidonic acid metabolites generated following stimulation of neuroinflammatory calcitonin gene-related peptide receptor, and to quantitate the amount of prostaglandin compounds hypothesized to be produced from an increased expression of cyclooxygenase-2. In Project 3 (Picklo), mass spectrometry is being used to study mechanisms of 4-hydroxy-2(E)-nonenal (HNE) methabolism in the central nervous system tissue, electrospray-triple quadrupole mass spectrometer will be used to identify and quantitate HNE methabolites, and electrospray-quadrupole/time-of-flight mass spectrometer will be used to identify novel detoxification enzymes. In Project 4 (Murphy), electrospray-triple quadrupole and gas chromatography-ion trap mass spectrometer is being used to measure phospholipids head group flux and turnover in neuronal, astroglia, and stably transfected HEK-293 cell lines, and to measure labeled and unlabeled phospholipids to study the kinetics of compound incorporation. The core facility consists of three instruments; electrospray-quadrupole/time-of-flight, electrospray-triple quadrupole and gas chromatography-ion trap mass spectrometer. These instruments provide us a capability of determining chemical structures of a wide variety of compounds generated in this COBRE as well as a capability of quantifying the compounds. The facility responsibilities (training users and helping with data analysis, operation and maintenance, assuring that the standard operating procedures are followed) will be divided between the facility director and the research assistant.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这一核心设施支持科布雷调查人员的研究。具体地说，高分辨率电喷雾四极杆/飞行时间质谱仪正在项目2(Porter)中用于鉴定刺激神经炎性降钙素基因相关多肽受体后产生的促炎花生四烯酸代谢物，并对假设由环氧合酶-2表达增加而产生的前列腺素化合物的数量进行定量。在项目3(Picklo)中，质谱仪将用于研究中枢神经系统组织中4-羟基-2(E)-壬烯醛(HNE)的代谢机制，电喷雾-三重四极杆质谱仪将用于鉴定和定量HNE代谢产物，电喷雾-四极杆/飞行时间质谱仪将用于鉴定新的解毒酶。在项目4(Murphy)中，使用电喷雾-三重四极杆和气相色谱-离子陷阱质谱仪测量了神经元、星形胶质细胞和稳定转染的HEK-293细胞系中磷脂头部基团的通量和周转，并测量了标记和未标记的磷脂，以研究化合物掺入的动力学。核心设备由三台仪器组成：电喷雾-四极杆/飞行时间、电喷雾-三重四极杆和气相色谱-离子陷阱质谱仪。这些仪器使我们能够确定在科布雷产生的各种化合物的化学结构，以及对化合物进行量化的能力。设施的责任(培训用户并协助数据分析、操作和维护，确保遵守标准操作程序)将由设施主任和研究助理分担。