COBRE: UND: MASS SPECTROMETRY CORE FACILITY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。这个核心设施支持COBRE调查人员的研究。具体而言，高分辨率电喷雾四极杆/飞行时间质谱仪正在项目2（Porter）中用于鉴定刺激神经炎性降钙素基因相关肽受体后产生的促炎性花生四烯酸代谢物，并定量假设由环氧合酶-2表达增加产生的前列腺素化合物的量。在项目3（Picklo）中，质谱法用于研究4-羟基-2（E）-壬烯醛（HNE）蛋氨酸在中枢神经系统组织中的作用机制，电喷雾-三重四极杆质谱仪将用于鉴定和定量HNE蛋氨酸，电喷雾-四极杆/飞行时间质谱仪将用于鉴定新型解毒酶。在项目4（Murphy）中，使用电喷雾-三重四极杆和气相色谱-离子阱质谱仪测量神经元、星形胶质细胞和稳定转染的HEK-293细胞系中的磷脂头基通量和转换，并测量标记和未标记磷脂，以研究化合物掺入的动力学。核心设施包括三种仪器：电喷雾-四极杆/飞行时间、电喷雾-三重四极杆和气相色谱-离子阱质谱仪。这些仪器为我们提供了确定COBRE中产生的各种化合物的化学结构的能力，以及量化化合物的能力。设施的责任（培训用户和帮助数据分析、操作和维护，确保遵守标准操作程序）将由设施主任和研究助理分担。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core facility supports the research of the COBRE investigators. Specifically, high-resolution electrospray-quadrupole/time-of-flight mass spectrometer is being used in Project 2 (Porter) for identification of pro-inflammatory arachidonic acid metabolites generated following stimulation of neuroinflammatory calcitonin gene-related peptide receptor, and to quantitate the amount of prostaglandin compounds hypothesized to be produced from an increased expression of cyclooxygenase-2. In Project 3 (Picklo), mass spectrometry is being used to study mechanisms of 4-hydroxy-2(E)-nonenal (HNE) methabolism in the central nervous system tissue, electrospray-triple quadrupole mass spectrometer will be used to identify and quantitate HNE methabolites, and electrospray-quadrupole/time-of-flight mass spectrometer will be used to identify novel detoxification enzymes. In Project 4 (Murphy), electrospray-triple quadrupole and gas chromatography-ion trap mass spectrometer is being used to measure phospholipids head group flux and turnover in neuronal, astroglia, and stably transfected HEK-293 cell lines, and to measure labeled and unlabeled phospholipids to study the kinetics of compound incorporation. The core facility consists of three instruments; electrospray-quadrupole/time-of-flight, electrospray-triple quadrupole and gas chromatography-ion trap mass spectrometer. These instruments provide us a capability of determining chemical structures of a wide variety of compounds generated in this COBRE as well as a capability of quantifying the compounds. The facility responsibilities (training users and helping with data analysis, operation and maintenance, assuring that the standard operating procedures are followed) will be divided between the facility director and the research assistant.