Noninvasive monitoring of physiological parameters in mice

无创监测小鼠生理参数

• 批准号: 7455714
• 负责人: LARRY D SANFORD
• 金额: $ 16.13万
• 依托单位: EASTERN VIRGINIA MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7455714
• 关键词: Affect; Animal Behavior; Animal Model; Animals; Arousal; Basic Science; Behavior; Behavioral; Biomedical Engineering; Body Surface; Body Temperature; Breathing; Caring; Collaborations; Collection; Computer software; Data; Development; Devices; Discrimination; Electrodes; Electroencephalography; Genes; Genetic; Genotype; Goals; Heart Rate; Human; Image; Implant; Implantation procedure; Informatics; Invasive; Knowledge; Laboratory Research; Measurement; Measures; Medical; Methodology; Methods; Modeling; Monitor; Movement; Mus; Numbers; Operative Surgical Procedures; Physiologic Monitoring; Physiologic pulse; Physiological; Physiology; Procedures; Public Health; Pulsar; Pulse taking; Qualifying; Radar; Rate; Rattus; Records Controls; Research; Research Project Grants; Respiratory Diaphragm; Rodent; Score; Signal Transduction; Sleep; Stress; Techniques; Technology; Temperature; Ultrasonics; Wakefulness; Whole Body Plethysmography; base; experience; improved; novel; research and development; respiratory; response; restraint; roentgen equivalent man; sensor; size

DESCRIPTION (provided by applicant): The use of animal models has been a major factor in the advancement of scientific and medical knowledge in virtually every biomedical field. Recent advances in genetics have provided the potential for even better models, particularly in gene-altered mice and rats. To fully exploit the potential of these models, the animals need to be evaluated on physiology as well as behavior and genotype. Unfortunately, the size of some rodents makes it difficult to use some established methods of recording physiological signals that involve invasive procedures and the implantation of electrodes. In addition, while these techniques have yielded valuable information regarding mechanisms, there are a number of limitations with applying them to behavioral questions. These include potential stress on the animal from surgery or restraint and the possibility that the recording technique can affect the parameter being measured, or the animals behavior. Current techniques that require surgery also may involve considerable labor in preparing and caring for chronically implanted animals. These limitations indicate the need for non-invasive techniques that can record physiological parameters. Our goal is to develop methodologies for non-invasive, non-contact monitoring of physiology and behavior in rodent animal models, and to assess their utility in determining arousal state (wakefulness and sleep). To accomplish these goals we will develop sensor applications and software for the non-invasive collection of heart rate, respiratory rate, body surface temperature and activity in rodents. We will assess pulse Doppler radar and/or ultrasonic sensors for acquiring heart rate, respiratory rate and activity, and we will develop infrared thermal imaging applications for acquiring body temperature. We will then validate non-invasively collected data for heart rate, respiratory rate, temperature and activity with data concurrently collected with established techniques. We will compare the data obtained with the sensor applications we develop with that obtained with established methodologies (e.g., telemetrically recorded heart rate, whole body plethysmography and diaphragm recordings for respiratory rate, telemetrically recorded temperature, and photobeam measurement of activity). Finally, we will determine if these signals can be used to reliably determine three arousal states (wakefulness, NREM and REM) by comparing state discrimination with non- invasively obtained signals to EEG based scoring of arousal states. This project has significance for public health because it can provide improved techniques for studies using animal models. The proposed applications have particular significance for studies of behavior and sleep.

描述（由申请人提供）：在几乎所有生物医学领域，动物模型的使用一直是科学和医学知识进步的主要因素。遗传学的最新进展为更好的模型提供了可能性，特别是在基因改变的小鼠和大鼠中。为了充分利用这些模型的潜力，需要对动物的生理、行为和基因型进行评估。不幸的是，一些啮齿类动物的体型使其难以使用一些现有的记录生理信号的方法，这些方法涉及侵入性手术和植入电极。此外，虽然这些技术已经产生了关于机制的有价值的信息，但将它们应用于行为问题有许多限制。这些包括手术或约束对动物的潜在压力，以及记录技术可能影响被测量参数或动物行为的可能性。目前需要手术的技术在准备和照顾长期植入的动物时也可能涉及相当大的劳动。这些限制表明需要非侵入性技术来记录生理参数。我们的目标是开发非侵入性、非接触式监测啮齿动物生理和行为的方法，并评估其在确定唤醒状态（清醒和睡眠）方面的效用。为了实现这些目标，我们将开发传感器应用和软件，用于无创收集啮齿动物的心率、呼吸频率、体表温度和活动。我们将评估脉冲多普勒雷达和/或超声波传感器用于获取心率，呼吸频率和活动，我们将开发红外热成像应用于获取体温。然后，我们将验证非侵入性收集的心率、呼吸频率、体温和活动数据，并同时使用已建立的技术收集数据。我们将把获得的数据与我们开发的传感器应用程序与已建立的方法获得的数据进行比较（例如，遥测记录的心率，全身体积脉搏图和呼吸频率的隔膜记录，遥测记录的温度，以及光束测量的活动）。最后，我们将通过将非侵入性获得的状态识别信号与基于脑电图的唤醒状态评分进行比较，来确定这些信号是否可以可靠地用于确定三种唤醒状态（清醒、非快速眼动和快速眼动）。该项目对公共卫生具有重要意义，因为它可以为使用动物模型的研究提供改进的技术。提出的应用对行为和睡眠的研究具有特别的意义。