NEUROENDOCRINE & GONADAL CONTROL OF MALE REPRODUCTION

神经内分泌

• 批准号: 6636858
• 负责人: William Francis Crowley
• 金额: $ 104.82万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6636858
• 关键词: cooperative study; male reproductive system; neuroendocrine system; reproductive development

DESCRIPTION: (Adapted from the applicant's description) This application for the Harvard-wide Specialized Center for Reproduction proposes to bring the neuroendocrine and gonadal control of reproduction in the male. Two of the component of reproduction while the other two will explore the gonadal level as Leydig cell control of steroidogenesis. In Project I, will employ tandem phenotyping and genotyping approaches in patients with idiopathic hypogonadotropic hypogonadism (IHH). This is a human model in which a series of genetic defects result in failure of the neuroendocrine component of sexual maturation. In Project II, Dr. Ursula Kaiser will explore the GnRH of gonadotropic genes using LH-T2 cells which are of gonadotrope origin. In Inhibiting Substance (MIS) upon steroidogenesis using the CYP17 gene as well MIS receptor. In addition, she will chart the developmental expression of this receptor and correlate it with steroidogenesis. In Project IV, Dr. Joel Habener will continue his explorations of the role of cAMP-activated pathways of alternative splicing of the gene encoding of the transcription factor, CREB. He will also explore the role of pituitary adenyl cyclase activating peptide; PACAP, and its alternatively spliced type 1 receptor in the autocrine control of spermatogenesis.

描述：（改编自申请人的描述）本申请申请哈佛大学生殖专业中心，旨在研究男性生殖的神经内分泌和性腺控制。其中两个组成部分的生殖，而另外两个将探讨性腺水平作为间质细胞控制的甾体发生。在项目1中，将采用串联表型和基因分型方法对特发性促性腺功能减退症（IHH）患者进行分析。这是一个人类模型，其中一系列遗传缺陷导致性成熟的神经内分泌成分失败。在Project II中，Dr. Ursula Kaiser将使用促性腺激素来源的LH-T2细胞来探索促性腺激素基因的GnRH。利用CYP17基因和MIS受体抑制物质（MIS）对类固醇生成的影响。此外，她将绘制该受体的发育表达图，并将其与类固醇形成联系起来。在项目IV中，Joel Habener博士将继续探索camp激活的转录因子CREB基因编码的选择性剪接途径的作用。他还将探讨垂体腺苷环化酶激活肽的作用；PACAP及其选择性剪接的1型受体在自分泌控制精子发生中的作用。