MICROSCOPY IMAGE ANALYSIS AND VISUALIZATION

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The National Center for Microscopy and Imaging Research (NCMIR), headed by Dr. Mark Ellisman at the University of California San Diego, is an NCRR NIH Biomedical Technology Research Center established to develop advanced, computer- aided microscopy for acquisition of structural and functional data. The goal of the CIBC collaboration with NCMIR is to develop image analysis algorithms and software to help biomedical researchers understand structural and functional relationships within cells and tissues through a range of scales from macromolecular complexes to organelles and multi- component structures like synapses. Several of our other collaborators have identified the extraction and analysis of cell geometry from microscopy data as a significant hurdle in their research, so the tools we develop with NCMIR are expected to be immediately useful to other researchers. We feel that microscopy data has not received the same level of attention from the image processing community as many of the more clinically oriented imaging modalities and offers unique and important challenges. With the NCMIR group, for example, we are developing tools for quantitative and subjective analysis of the structural properties of dendritic spines and mitochondrial structure, a challenging project that requires segmentation of reconstructed electron microscope tomography volumes and research into new volume visualization techniques.