Connecting the dots: Image analysis for single molecule localisation microscopy

连接点：单分子定位显微镜图像分析

• 批准号: LP140100967
• 负责人: Prof Katharina Gaus
• 金额: $ 27.23万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-09-25 至 2019-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP140100967
• 关键词: Connecting; dots; Image; analysis; single

It is now possible to record the positions of single fluorescent molecules in intact cells to build up an image literally molecule by molecule. But how to ‘connect the dots’ and extract structural information from molecular coordinates is yet to be worked out. The project aims to do exactly that, and write and implement novel analysis routines to quantify a diverse range of biological structures such as protein complexes, membrane morphologies, filamentous cytoskeletal networks, vesicles and viruses. The project is a collaboration between a cell biologist (Professor Gaus) who is a leader in single molecule localisation microscopy, an expert in fluorescence image analysis (Dr Nicovich) and an industry partner (Dr Lucas) who has a sophisticated software platform.

现在可以记录完整细胞中单个荧光分子的位置，从而逐个分子地建立图像。但是如何“连接点”并从分子坐标中提取结构信息还有待解决。该项目旨在做到这一点，并编写和实施新的分析程序，以量化各种生物结构，如蛋白质复合物，膜形态，丝状细胞骨架网络，囊泡和病毒。该项目是细胞生物学家（Gaus教授），他是单分子定位显微镜的领导者，荧光图像分析专家（Nicovich博士）和拥有复杂软件平台的行业合作伙伴（Lucas博士）之间的合作。