Request for SCANCO micro-CT 40 Multi-User Bone Studies

索取 SCANCO micro-CT 40 多用户骨骼研究

• 批准号: 7389778
• 负责人: Jane B. Lian
• 金额: $ 27.67万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7389778
• 关键词: 3-Dimensional; Address; Animals; Area; Basic Science; Biocompatible Materials; Biology; Bone Density; Bone Diseases; Bone Regeneration; Bone Tissue; Clinical Trials; Data; Defect; Degenerative polyarthritis; Diagnostic radiologic examination; Dimensions; Faculty; Fracture; Funding; Genetic; Goals; Grant; Healed; Histology; Image; Magnetic Resonance Imaging; Measurement; Mechanics; Medical; Methods; Modeling; Mus; Nature; Numbers; Osteogenesis; Osteolytic; Osteoporosis; Porosity; Property; Rattus; Recruitment Activity; Research; Research Personnel; Resolution; Skeletal system; Specimen; Standards of Weights and Measures; Structure; System; Techniques; Technology; Testing; Thick; Tissues; Translational Research; United States National Institutes of Health; X-Ray Computed Tomography; abstracting; anticancer research; bone; healing; instrument; instrumentation; irradiation; medical schools; mouse model; novel strategies; novel therapeutics; programs; repaired; tumor growth

DESCRIPTION (provided by applicant): Project Summary/Abstract: The goal of this proposal is to acquire funds to purchase a Scanco <CT40 instrument for 2- and 3-dimensional imaging and quantitative analyses of trabecular and cortical bone properties of small animal (mouse and rat) models. This instrument is to be shared among 15 users (10 are supported by 14 NIH grants, and 5 are recently recruited junior faculty). There is no similar instrument available on the UMASS Medical School, Worcester campus and at present sixteen research programs are in need of this for quantitative assessment of skeletal abnormalities in genetic mouse models, addressing novel therapeutic approaches for osteoarthritis and defects in fracture repair, characterization of new biomaterial substitutes for bone repair, and novel approaches for protecting the bone from irradiation damage and the osteolytic destruction caused by tumor growth in bone. Currently, these projects use multiple methods including standard radiography, magnetic resonance imaging, and histology to assess tissue structure and tissue healing. Micro-computed tomography adds an important dimension to this existing armamentarium by providing capabilities for detailed characterization of bone tissue by quantitative measurements such as bone mineral density, trabecular and cortical thickness, bone porosity and other parameters that reflect bone formation and resorption. The <CT data is critical in elucidating relationships between bone structure and function in both healthy and diseased tissues. The nondestructive nature of <CT imaging allows subsequent analysis of a single specimen by any number of complimentary techniques, including histology and mechanical testing. Thus, use of <CT does not require an increase in specimen or animal numbers. Our previous use of <CT has been limited by access to this instrumentation, rather than the utility of this technology for our research. A goal in obtaining the <CT is to facilitate translational research that is a major direction of the UMASS Medical campus in basic research and clinical investigations are becoming well integrated in the areas or skeletal biology and cancer research. The high-resolution desktop <CT system requested in this application will be fully utilized by a team of investigators who have a strong track record of providing important results, as well as new research directions in skeletal healing, osteoporosis, osteoarthritis and metastatic bone disease.

描述（由申请人提供）： 项目摘要/摘要：本提案的目标是获得资金购买 Scanco <CT40 仪器，用于对小动物（小鼠和大鼠）模型的小梁和皮质骨特性进行 2 维和 3 维成像和定量分析。该仪器将由 15 名用户共享（10 名用户得到 14 项 NIH 拨款的支持，5 名是最近招募的初级教师）。马萨诸塞大学伍斯特分校医学院没有类似的仪器，目前有 16 个研究项目需要这种仪器来定量评估遗传小鼠模型中的骨骼异常，解决骨关节炎和骨折修复缺陷的新治疗方法，骨修复的新生物材料替代品的特性，以及保护骨骼免受辐射损伤和溶骨性破坏的新方法 由骨内肿瘤生长引起。目前，这些项目使用多种方法，包括标准放射线照相、磁共振成像和组织学来评估组织结构和组织愈合。微计算机断层扫描通过定量测量（例如骨矿物质密度、小梁和皮质厚度、骨孔隙率以及反映骨形成和吸收的其他参数）提供对骨组织进行详细表征的能力，为现有的设备增加了一个重要维度。 CT 数据对于阐明健康组织和患病组织的骨结构和功能之间的关系至关重要。 <CT 成像的无损性质允许通过任意数量的补充技术（包括组织学和机械测试）对单个样本进行后续分析。因此，使用<CT不需要增加样本或动物数量。我们之前对 CT 的使用受到了该仪器的限制，而不是该技术对我们研究的实用性。获得<CT的目标是促进转化研究，这是马萨诸塞大学医学院基础研究和临床研究的一个主要方向，正在与骨骼生物学和癌症研究领域很好地结合起来。本申请中要求的高分辨率桌面<CT系统将由一组研究人员充分利用，他们在提供重要结果以及骨骼愈合、骨质疏松症、骨关节炎和转移性骨疾病方面的新研究方向方面有着良好的记录。

项目成果

Age-dependent Changes in the Articular Cartilage and Subchondral Bone of C57BL/6 Mice after Surgical Destabilization of Medial Meniscus.

• DOI: 10.1038/srep42294
• 发表时间: 2017-02-09
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Huang H;Skelly JD;Ayers DC;Song J
• 通讯作者: Song J