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Alcohol & FAS-Mediated Apoptosis in Polarized Liver Cell

酒精

基本信息

批准号：
7337638
负责人：
BENITA L. MCVICKER
金额：
$ 10.98万
依托单位：
UNIVERSITY OF NEBRASKA MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-02-01 至 2011-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7337638
关键词：
Activation Analysis Alcohols Apoptosis Apoptotic Architecture Area B-Lymphocytes Biological Assay Biological Models CD95 Antigens Cell Death Cell Line Cell hybridization Cell membrane Cell model Cell physiology Cell surface Cells Cessation of life Characteristics Condition Consultations District of Columbia Doctor of Philosophy Dose Ethanol Evaluation Event Fibroblasts Flow Cytometry Future Gene Expression Generations Goals Grant Hepatocyte Hybrid Cells Instruction Knowledge Laboratories Lead Ligands Liver Location Mediating Medical center Membrane Mentors Methods Microinjections Modeling Nebraska Pathway interactions Phenotype Phosphorylation Play Primary carcinoma of the liver cells Property Proteins Receptor Signaling Research Role Signal Transduction Study models System TNFRSF6 gene Techniques Time Trace Elements Training Training Support Universities Vesicle Work Zinc alcohol abuse therapy alcohol effect bile canaliculus structure cell injury chronic alcohol ingestion design experience functional mimics in vivo inhibitor/antagonist interest intracellular protein transport liver function protein transport receptor research study tool trafficking

项目摘要

Benita L. McVicker received her PhD from The University of Nebraska Medical Center (UNMC) in 1997 and has worked in the laboratories of Drs. Dean Tuma and Carol Casey in the Liver Study Unit at the VA Medical Center in Omaha, Nebraska since that time. The Liver Study Unit has been instrumental in establishing that chronic ethanol consumption can lead to a variety of pathological consequences, yet further clarification of the mechanism(s) by which ethanol causes hepatotoxic conditions is required. Recently, Dr. McVicker and co-workers have identified the use of a fibroblast/hepatoma hybrid cell line (WIF-B) as a model for studying the effects of ethanol on cellular processes. The generation of such a physiologically important polarized model (that cannot be accomplished with regular isolated hepatocytes) will enable the evaluation of protein trafficking events (Fas/CD95 death receptor) in the presence of alcohol in relation to alterations in hepatocyte polarity and apoptosis. Specifically, the goals of Dr. McVicker's research presented in this grant include 1) further characterization of ethanol's effects on Fas trafficking and 2) to identify the role of specific regulators that are involved in ethanol-induced Fas translocation and the correlation of these effects with Fas-induced apoptosis mechanisms. The study of death receptor trafficking and signaling as well as the use of the WIF-B model are new areas of interest to Dr. McVicker. For these studies, she has proposed to use several techniques (i.e.immunohistochemistry and microinjection assays) that will require additional training and specialized instruction. Dr. McVicker has access to the confocal and flow cytometry core labs at UNMC and has support for training consultations in those methods. In addition, she has support to leam specific techniques under the direction of Dr. Pamela Tuma (an expert in the use of WIF-B cells) at The Catholic University in Washington D.C. Overall, the completion of this proposed work will provide the mentored experience to progress to future independent study of liver function and survival following ethanol treatment.

Benita L. McVicker于1997年获得内布拉斯加州大学医学中心（UNMC）的博士学位