DNA Sequencing

DNA测序

• 批准号: 7697010
• 负责人: Beatrice H Hahn
• 金额: $ 12.28万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-16 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7697010
• 关键词: Acquired Immunodeficiency Syndrome; Arts; Back; Base Pairing; Base Sequence; Bioinformatics; Budgets; Capillary Electrophoresis; Charge; Computer Analysis; Computer software; Computers; Contracts; DNA; DNA Sequence; DNA Sequence Analysis; DNA Sequencing Facility; Databases; Development; Funding; Gel; Genes; Genomics; Goals; Grant; HIV; Immune response; Maintenance; Methodology; Molecular; Molecular Genetics; Numbers; Operative Surgical Procedures; Pathogenesis; Pharmaceutical Preparations; Preparation; Process; Research; Research Personnel; Resources; Sequence Analysis; Sequence Determination; Services; System; Training; Training Support; Vaccines; analytical tool; design; drug discovery; gene function; genetic analysis; member; multidisciplinary; programs; rapid technique; success; vaccine development

The UAB CFAR DNA Sequence and Analysis Core, which is comprised of a DNA Sequencing Facility and a Molecular and Genetic Bioinformatic Facility, has been in operation for the past fourteen years and has provided users with state-of-the-art automated sequencing and computer analyses capabilities. The specific goals of the Sequence and Analysis Core are: 1. To provide state-of-the-art automated DNA sequencing capabilities to AIDS Center members through the availability and maintenance of dedicated Applied Biosystems DNA Sequencers using both slab gel and capillary electrophoresis systems. 2. To provide technical support and training in template preparation, primer design, cycle sequencing methodologies, and sequence editing. 3. To provide and maintain a comprehensive set of modern bioinformatic databases and analytical tools for the analysis of genetic information, including gene and genomic sequences. 4. To provide technical support and training in the use of these bioinformatic resources. In the past budget period, the DNA Sequence and Analysis Core has supported over 145 funded grants and contracts for 120 CFAR investigators. The DNA Sequencing Facility generated over five million base pairs of primary sequence and the Molecular and Genetic Bioinformatic Facility trained and provided logistical support for 62 different projects. In the process of providing these services, the core has generated $259,814 of charge-backs. Over 75 funded projects as well as several grants currently under review are absolutely dependent on the availability of automated DNA sequencing and sophisticated sequence analysis. Support of the DNA Sequence and Analysis Core is thus instrumental for the continuing success of the numerous HIV/SIV pathogenesis, vaccine and drug studies currently conducted at UAB.

UAB CFAR DNA测序和分析核心，由DNA测序设施组成 以及一个分子和遗传生物信息学设施，已经运作了14年， 为用户提供了最先进的自动测序和计算机分析能力。的 测序和分析核心的具体目标是： 1.为艾滋病中心成员提供最先进的自动DNA测序能力， 专用的应用生物系统DNA测序仪的可用性和维护， 和毛细管电泳系统。 2.在模板制备、引物设计、循环测序等方面提供技术支持和培训 方法和序列编辑。 3.提供和维护一套全面的现代生物信息学数据库和分析工具 用于分析遗传信息，包括基因和基因组序列。 4.在使用这些生物信息资源方面提供技术支持和培训。 在过去的预算期间，DNA测序和分析核心项目支持了超过145项资助赠款 和120名CFAR调查员的合同。DNA测序设备产生了超过五百万个碱基 对一级序列和分子和遗传生物信息学设施的培训和提供 为62个不同项目提供后勤支持。在提供这些服务的过程中， 259,814美元的退款。超过75个资助项目以及目前正在审查的几项赠款， 完全依赖于自动化DNA测序和复杂的序列 分析.因此，对DNA测序和分析核心的支持有助于 UAB目前进行的大量HIV/SIV发病机制、疫苗和药物研究。