FUNCTIONALITY CORE

功能核心

基本信息

批准号：
7404522
负责人：
JOHN Arthur FAULKNER
金额：
$ 8.93万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-05-01 至 2008-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7404522
关键词：
Activities of Daily Living Age Aging Animals Area Arts Cardiac Cardiac Myocytes Cells Complex Consultations Development Disease Drosophila genus Elderly Electronics Equipment Facility Construction Funding Category Faculty Fiber Gene Transfer Goals Heart Housing Human Resources Impairment In Situ In Vitro Injury Isometric Exercise Laboratories Limb structure Measurement Measures Mechanics Microfilaments Mus Muscle Muscle Fibers Mutant Strains Mice Myocardium Myofibrils Myopathy Myosin ATPase Organ Organism Pattern Play Postdoctoral Fellow Predisposition Property Protocols documentation Purpose Quality of life Range Rattus Research Personnel Respiratory Diaphragm Rodent Role Sarcomeres Services Shock Skeletal Muscle Skeletal system Stretching Striated Muscles Structure Structure-Activity Relationship Students Techniques Testing Training Transgenic Mice Transgenic Organisms Work base design fiber cell gene therapy genetic manipulation heart function hemodynamics in vivo innovation mutant programs success vector

项目摘要

For striated muscles, functionality is defined as the capability of an organ, the heart or a muscle, or a cell, muscle fibers, to develop force and power during isometric, shortening, or lengthening contractions. Our working hypothesis is that functionality of striated muscles is a complex phenomenon and the provision of reliable and valid measurements requires sophisticated equipment and highly trained muscle mechanists to make the measurements and analyze and interpret the results. Consequently, to test rigorously hypotheses related to the underlying mechanisms of the reduced functionality of striated muscles in diseased and old animals, a Functionality Core is a necessity. The specific aims of the Functionality Core are: (1) the provision of valid, state-of-the-art, functional measurements: (1a) for Project #1 of single permeabilized fibers of limb muscles and whole limb muscles of wild type, dystrophic, transgenic and mutant adult and old mice, and in mice after vector-based gene transfer; (1b) for Project #2 of single cardiac myocyte function and whole heart hemodynamics of adult and old, wild type and mutant mice, and in young and old mice and rats after vector-based gene transfer in vitro and in vivo; (1c) for Project #3 of single permeabilized fibers from flight muscles, force and power of flight muscles during tethered flight, and whole heart muscle functionality of wild type, transgenic and mutant Drosophila; (2) continuous up-dating of the design, development, and construction of innovative experimental protocols and apparatus to measure specific mechanical properties of striated muscles; (3) consultation and training of trainees and faculty to make measurements of functionality and maintain the apparatus used to measure functional properties of striated muscles of rodents and Drosophila. The significance of the Functionality Core lies in the critical role that functional properties play in the interpretation of the structure-function relationships with natural aging of the organism and following any genetic manipulation of skeletal or cardiac muscles of rodents or Drosophila. Impairments in functionality of striated muscles may occur at any age due to injury or disease or as an intractable concomitant of aging. The impairments in functionality, whether due to injury, disease, or old age, limit the activities of daily living and reduce the quality of life.

对于横纹肌肉，功能定义为器官，心脏或肌肉或细胞，肌肉纤维的能力，在等距，缩短或延长收缩期间会发展力和功率。我们的工作假设是，横纹肌肉的功能是一种复杂的现象，提供可靠和有效的测量结果需要复杂的设备和训练有素的肌肉机械师来进行测量并分析和解释结果。因此，要测试与患病和旧的肌肉功能降低功能的基本机制有关的严格假设动物，功能核心是必要的。功能核心的具体目的是：（1）提供有效的，最先进的功能测量值：（1a）肢体肌肉的单个通透性纤维的项目＃1，野生型，营养不良，转基因和突变的成人和旧小鼠的野生型肌肉以及整个野生型肌肉，以及在载体基于载体的基于基于载体的基于基于基于的基于基于基于的基因基因的基因转移；（1B）对于单个心肌细胞功能的项目＃2以及成人和老年人和突变小鼠的整个心脏血液动力学，以及在基于矢量的基因转移后体外和体内的年轻和老鼠和大鼠中；（1C）用于从束缚飞行过程中飞行肌肉，飞行肌肉力量的单个通透纤维的项目＃3，以及野生型，转基因和突变果蝇的全心肌功能；（2）持续的设计，开发和构建创新的实验方案和设备，以测量横纹肌肉的特定机械性能；（3）对学员和教职员工进行咨询和培训，以测量功能，并保持用于测量啮齿动物和果蝇肌肉肌肉的功能特性的设备。功能核心的重要性在于关键作用是功能性能在解释与生物体自然衰老的结构 - 功能关系中起着作用，并在对啮齿动物或果蝇的骨骼或心脏肌肉进行任何遗传操纵之后。由于受伤或疾病或顽固的衰老伴随，横纹肌肉功能的障碍可能在任何年龄发生。功能障碍，无论是由于伤害，疾病还是老年，都限制了日常生活的活动并降低生活质量。