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The Yersinia insecticidal toxin complex

耶尔森氏菌杀虫毒素复合物

基本信息

批准号：
7498011
负责人：
Samuel I Miller
金额：
$ 22.86万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-30 至 2010-08-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Plague, or the Black Death, is a zoonotic disease that is spread from mammal to mammal by fleas. This mode of transmission demands that the causative agent of this disease, Yersinia pestis, is able to circumvent the host defense systems of both mammals and insects. Much is known about the interaction of Y. pestis with the mammalian host, however, there is only limited understanding of Y. pestis determinants that allow survival and replication in the insect. In recent years the complete genome sequence of Y. pestis has been determined. This sequence information indicates that Y. pestis contains a cluster of genes with significant homology to insecticidal toxin encoding genes of the insect pathogen Photorhabdus luminescens. The goals of this proposal are to determine if this Y. pestis insecticidal complex increases the virulence of this pathogen, either by resulting in increase colonization of the flea or increased transmission of the bacteria from the flea to a mammal. In addition, initial studies to examine the activity of this complex, and the mechanism of secretion of this complex are proposed. Plague is an epidemic disease of antiquity that continues today with approximately 2,000 cases per year. The causative agent Yersinia pestis is maintained in various rodent species across the world and spread via their respective flea vectors. The goals of this proposal are to examine the role of a newly found toxin complex in Y. pestis flea colonization and/or transmission.

描述(申请人提供)：鼠疫，或黑死病，是一种通过跳蚤在哺乳动物之间传播的人畜共患疾病。这种传播方式要求这种疾病的病原体--鼠疫耶尔森氏菌--能够绕过哺乳动物和昆虫的宿主防御系统。关于鼠疫杆菌与哺乳动物宿主的相互作用，人们知道的很多，但对允许在昆虫中生存和复制的鼠疫杆菌决定因素的了解有限。近年来，鼠疫耶尔森氏菌的全基因组序列已经确定。这些序列信息表明，鼠疫耶尔森氏菌包含一组与昆虫病原体发光菌的杀虫毒素编码基因有显著同源性的基因。这项提议的目标是确定这种鼠疫杀虫复合体是否通过增加跳蚤的定殖率或增加细菌从跳蚤到哺乳动物的传播来增加这种病原体的毒力。此外，还对该复合体的活性和分泌机制进行了初步研究。鼠疫是一种古老的流行病，至今仍在继续，每年约有2000例。鼠疫耶尔森氏菌存在于世界各地的各种啮齿动物中，并通过各自的跳蚤媒介传播。这项建议的目标是研究新发现的毒素复合体在鼠疫跳蚤定植和/或传播中的作用。