Funtion of Uncharacterized Genes of Acinetobacter baumanii

描述（由申请人提供）：鲍曼不动杆菌（Ab）是一种引起原发性和机会性感染的新兴病原体，在医院和战场环境中引起严重关注。这种微生物对抗生素具有显著的内在抗性，并能够迅速获得新的抗性。此外，Ab可以在污染物上以干燥状态持续存在，使得医院感染的消毒和管理特别麻烦。 本研究的目的是通过阐明Ab的非特征基因在该生物体的发病机制、抗性和持久性中的作用来找到未开发的治疗方法。 将建立三个项目，1）使用基因组方法广泛识别未表征基因的作用（Colin Manoil，项目1主任）2）确定包膜完整性对细菌活力的作用（Sam Mille，PI/PD，项目2总监），并使用表达分析来鉴定与药物、消毒剂和干燥耐受性相关的未知功能的基因和sRNA（Carrie Harwood，项目3总监）该中心的工作将得到一个行政核心（核心1，John Kemner核心负责人），一个蛋白质相互作用技术核心（Jim布鲁斯核心2主任负责人）和一个数据传播/资源管理核心（Mitch Brittnacher，核心3主任）的支持。

DESCRIPTION (provided by applicant): Acinetobacter baumannii (Ab) is an emerging pathogen that causes primary and opportunistic infections, and is a serious concern in the hospital and battlefield setting. This organism has significant intrinsic resistance to antibioticsas well as an ability to quickly acquire new resistance. Additionally, Ab can persist in a desiccated state on fomites, making disinfection and management of nosocomial infections particularly troublesome. The goal of this research proposal is to find unexploited therapeutic approaches by elucidating the role of uncharacterized genes of Ab in this organism's mechanisms of pathogenesis, resistance, and persistence. Three projects will be established to 1) use a genomic approach to broadly identify the role of uncharacterized genes (Colin Manoil, Project 1 Director) 2) determine the role of envelope integrity with regard to bacterial viability (Sam Mille, PI/PD, Project 2 Director), and use expression analysis to identify genes and sRNAs of unknown function associated with drug, disinfectant, and desiccation tolerance (Carrie Harwood, Project 3 Director). The work of the Center will be supported by an Administrative Core (Core 1, John Kemner Core Leader), a Protein Interaction Technology Core (Jim Bruce Core 2 Director Leader), and a Data Dissemination/Resource Management Core (Mitch Brittnacher, Core 3 Director).