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Funtion of Uncharacterized Genes of Acinetobacter baumanii

鲍曼不动杆菌未表征基因的功能

基本信息

批准号：
9282562
负责人：
Samuel I Miller
金额：
$ 146.37万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-06-24 至 2019-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9282562
关键词：
Acinetobacter Acinetobacter baumannii Antibiotic Resistance Antibiotics Antimicrobial Resistance Bacteria Biochemical Biocide Cell Membrane Permeability Cell surface Code Communicable Diseases Data Desiccation Development Disinfectants Disinfection Drug Modelings Drug Targeting Drug resistance Enzymes Genes Genetic Genomic approach Genomics Goals Hospitals Individual Infection Knowledge Molecular Multi-Drug Resistance Natural Immunity Nosocomial Infections Opportunistic Infections Organism Pathogenesis Pharmaceutical Preparations Primary Infection Process Proteins Proteomics Research Research Proposals Resistance Resources Role Surface Properties Technology Testing Therapeutic Work antimicrobial biological adaptation to stress clinical phenotype clinically relevant data management efflux pump environmental change gene function genetic analysis genome-wide human disease mutant novel strategies pathogen personalized approach programs public health relevance relational database resistance gene response screening trait

项目摘要

DESCRIPTION (provided by applicant): Acinetobacter baumannii (Ab) is an emerging pathogen that causes primary and opportunistic infections, and is a serious concern in the hospital and battlefield setting. This organism has significant intrinsic resistance to antibioticsas well as an ability to quickly acquire new resistance. Additionally, Ab can persist in a desiccated state on fomites, making disinfection and management of nosocomial infections particularly troublesome. The goal of this research proposal is to find unexploited therapeutic approaches by elucidating the role of uncharacterized genes of Ab in this organism's mechanisms of pathogenesis, resistance, and persistence. Three projects will be established to 1) use a genomic approach to broadly identify the role of uncharacterized genes (Colin Manoil, Project 1 Director) 2) determine the role of envelope integrity with regard to bacterial viability (Sam Mille, PI/PD, Project 2 Director), and use expression analysis to identify genes and sRNAs of unknown function associated with drug, disinfectant, and desiccation tolerance (Carrie Harwood, Project 3 Director). The work of the Center will be supported by an Administrative Core (Core 1, John Kemner Core Leader), a Protein Interaction Technology Core (Jim Bruce Core 2 Director Leader), and a Data Dissemination/Resource Management Core (Mitch Brittnacher, Core 3 Director).

描述（由申请人提供）：鲍曼不动杆菌（Ab）是一种引起原发性和机会性感染的新兴病原体，在医院和战场环境中是一个严重的问题。这种生物对抗生素具有显著的内在抗性，并具有迅速获得新抗性的能力。此外，Ab可以在污染物上保持干燥状态，使医院感染的消毒和管理特别麻烦。本研究计划的目标是通过阐明未表征的Ab基因在该生物体的发病机制、耐药性和持久性中的作用，找到尚未开发的治疗方法。将建立三个项目：1)使用基因组方法广泛识别未表征基因的作用（Colin Manoil，项目1主任）；2)确定包膜完整性在细菌活力方面的作用（Sam Mille, PI/PD，项目2主任）；并使用表达分析识别与药物、消毒剂和干燥耐受性相关的未知功能的基因和sRNAs （Carrie Harwood，项目3主任）。该中心的工作将由行政核心（核心1，John Kemner核心领导），蛋白质相互作用技术核心（Jim Bruce核心2主任领导）和数据传播/资源管理核心（Mitch Brittnacher，核心3主任）提供支持。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Importance of Core Genome Functions for an Extreme Antibiotic Resistance Trait.

DOI：
10.1128/mbio.01655-17
发表时间：
2017-12-12
期刊：
mBio
影响因子：
6.4
作者：
Gallagher LA;Lee SA;Manoil C
通讯作者：
Manoil C

CsrA-Controlled Proteins Reveal New Dimensions of Acinetobacter baumannii Desiccation Tolerance.

CsrA 控制的蛋白质揭示了鲍曼不动杆菌干燥耐受性的新维度。

DOI：
10.1128/jb.00479-21
发表时间：
2022
期刊：
Journal of bacteriology
影响因子：
3.2
作者：
Oda,Yasuhiro;Shapiro,MadelynM;Lewis,NathanM;Zhong,Xuefei;Huse,HollyK;Zhong,Weizhi;Bruce,JamesE;Manoil,Colin;Harwood,CarolineS
通讯作者：
Harwood,CarolineS

Antibiotic Resistance and Regulation of the Gram-Negative Bacterial Outer Membrane Barrier by Host Innate Immune Molecules.