Stability of cross-linked dentin and its clinical application

交联牙本质的稳定性及其临床应用

• 批准号: 7434472
• 负责人: Ana Karina B Bedran-Russo
• 金额: $ 13.82万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-15 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7434472
• 关键词: Accounting; Adhesives; Affect; Amino Acids; Award; Biochemical; Biocompatible Materials; Biological Assay; Chemicals; Collagen; Collagen Type I; Composite Resins; Dental; Dental caries; Dentin; Doctor of Philosophy; Educational process of instructing; Elasticity; Endopeptidases; Environment; Evaluation; Failure; Funding; Goals; Grant; Hardness; High Pressure Liquid Chromatography; Human; Investigation; Label; Maintenance; Mechanics; Mentors; Modification; Molecular; Numbers; Object Attachment; Patient Care; Peptide Hydrolases; Procedures; Property; Research; Research Training; Resistance; Scanning Probe Microscopes; Scientist; Site; Structure; Techniques; Tensile Strength; Testing; Time; Tissue Engineering; Tissues; Tooth Components; Tooth structure; Training; Translational Research; Transmission Electron Microscopy; Water; career; clinical application; crosslink; improved; insight; multidisciplinary; nano; nanoscale; oral biology; oral condition; restoration; restoration placement; skills; sound

DESCRIPTION (provided by applicant): The long term goal of this proposal is to provide Dr. Ana Karina Bedran-Russo, DOS, MS, PhD with the opportunity to develop into a well prepared, competitive and independent young scientist that will combine an active research career with teaching and practice. The applicant's ultimate goal is to improve patient care by contributing to translational research. Additional training in tissue engineering, oral biology and biomaterials obtained during this K08 Mentored Clinician Scientist Award will provide the necessary skills for a dental academic career track within a supportive multidisciplinary environment for the rapid transition of Dr. Bedran- Russo to an independent clinician scientist. Her research and training will be conducted under the guidance of Dr. James Drummond and Dr. Anne George. The applicant will build upon this research to seek additional funding. The research component of this grant aims to investigate the contribution of biochemical modifications to collagen on the properties of dentin and consequently on the durability of adhesive restorative dental treatments. A composite resin restoration last an average of 7 years in posterior teeth at which time failure at the interface is the primary reason given for replacement and accounts for 30-60% of all replaced restorations. The integrity of fibrillar type I collagen is an important issue when bonding to dentin since it is the main tooth component involved during restorative procedures. The hypothesis to be tested is that the artificially formed collagen cross-links in dentin will result in restoration interfaces with improved mechanical properties to withstand degradation over time. The specific aims proposed are: Aim 1. Determine the biochemical properties of dentin following treatment with different crosslinking agents. Biochemical properties (collagen cross-linking analysis - HPLC and immunohistochemical analysis) will be assessed in sound and caries-affected human dentin in both short and long term evaluations. Aim 2: Assess the mechanical properties of the treated dentin (sound and caries-affected) at short-term and long-term evaluations. Stiffness and hardness will be evaluated in specific sites using an atomic force microscope. Ultimate strength of dentin and bond strength of the dentin/restoration interface will be assessed using a universal testing machine. These combined biochemical and mechanical approaches will provide insights to correlate quality of dentin collagen and the long-term durability of restorations.

描述（由申请人提供）：本提案的长期目标是为 Ana Karina Bedran-Russo 博士（DOS、MS、PhD）提供机会，使其发展成为一名准备充分、有竞争力且独立的年轻科学家，将积极的研究生涯与教学和实践相结合。申请人的最终目标是通过促进转化研究来改善患者护理。在 K08 指导临床科学家奖期间获得的组织工程、口腔生物学和生物材料方面的额外培训将为 Bedran-Russo 博士在支持性多学科环境中快速过渡为独立临床科学家提供牙科学术职业生涯所需的技能。她的研究和培训将在詹姆斯·德拉蒙德博士和安妮·乔治博士的指导下进行。申请人将在此研究的基础上寻求额外的资金。该赠款的研究部分旨在研究胶原蛋白的生化修饰对牙本质特性的影响，从而影响粘合修复牙科治疗的耐久性。复合树脂修复体在后牙中的平均使用寿命为 7 年，此时界面失效是更换的主要原因，占所有更换修复体的 30-60%。纤维状 I 型胶原蛋白的完整性在与牙本质粘合时是一个重要问题，因为它是修复过程中涉及的主要牙齿成分。待测试的假设是，牙本质中人工形成的胶原蛋白交联将导致修复体界面具有改善的机械性能，以承受随时间推移的降解。提出的具体目标是： 目标 1. 确定用不同交联剂处理后牙本质的生化特性。将在短期和长期评估中评估健康和受龋齿影响的人类牙本质的生化特性（胶原交联分析 - HPLC 和免疫组织化学分析）。目标 2：在短期和长期评估中评估经过处理的牙本质（健全的和受龋齿影响的）的机械性能。将使用原子力显微镜在特定部位评估刚度和硬度。将使用通用测试机评估牙本质的极限强度和牙本质/修复体界面的粘合强度。这些生物化学和机械方法相结合，将为将牙本质胶原蛋白的质量与修复体的长期耐久性联系起来提供见解。