PROTEIN BIOCHEMISTRY

蛋白质生物化学核心将提供最先进的蛋白质表达和 纯化、蛋白质-蛋白质相互作用研究、免疫沉淀和酶分析 在个别项目中追求的研究目标。通过在以下组织的领导下集中这些服务 两位经验丰富的调查员Patrick Sung(核心总监)和Steven Raynard(核心协调人)， 蛋白质生物化学核心将提高计划项目成员的生产力并促进协同 在不同的项目之间，通过简化关键试剂和方法的访问 实验室。 根据已建立的方案，蛋白质生物化学核心人员将在 细菌、酵母或昆虫细胞及蛋白质的提纯。此外，核心将与参与的 实验室开发新的蛋白质表达和纯化程序。帮助应对新的挑战 计划、核心主任和核心协调人将就有关事项与计划成员进行磋商 关于蛋白质-蛋白质相互作用的研究、免疫沉淀和酶的发展 化验。

The Protein Biochemistry Core will provide state-of-the-art technical support in protein expression and purification, protein-protein interaction studies, immunoprecipitation, and enzymatic assays germane for the research goals being pursued in individual projects. By centralizing these services under the leadership of two experienced investigators, Patrick Sung (Core Director) and Steven Raynard (Core Co-ordinator), the Protein Biochemistry Core will enhance the productivity of program project members and promote synergy among the different projects by streamlining access of critical reagents and methodologies across laboratories. Following established protocols, the Protein Biochemistry Core personnel will carry out protein expression in bacteria, yeast, or insect cells and protein purification. Moreover, the Core will work with the participating laboratories to develop new protein expression and purification procedures. To help meet new challenges of the program, the Core Director and Core Co-ordinator will consult with program members on matters pertaining to protein-protein interaction studies, immunoprecipitation, and the development of enzyme assays.