AN EXPERIMENTAL MODEL FOR CHORIOAMNIONITIS AND PREMATURITY

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 早产及其后果仍然是产科最重要的问题。 12%的早产新生儿占非先天性畸形新生儿死亡的80%。 宫内感染是早产的一个重要原因，也是可以预防的。 本研究的长期目标是：a）阐明感染诱导早产的病理生理机制; B）建立宫内感染和早产之间的细胞和分子联系; c）测试感染背景下早产的合理干预策略的有效性。 我们在妊娠恒河猴中开发了第一个实验模型，其中通过羊膜内接种已知浓度的B族链球菌（GBS）细菌建立宫内感染。 接种后，相继产生促炎性细胞因子、胰高血糖素和基质金属蛋白酶（MMP）。 然后，在所有情况下，子宫收缩，分娩和分娩，通常在72小时内。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Prematurity and its consequences remain the most important problem in obstetrics. The 12% of neonates born prematurely account for 80% of neonatal deaths not attributable to congenital malformations. Intrauterine infections are an important, and potentially preventable, cause of preterm delivery. The long-term objectives of this research are: a) to elucidate the pathophysiologic mechanisms of infection-induced preterm labor; b) to establish the cellular and molecular links among intrauterine infection and preterm labor; and c) to test the efficacy of rational interventional strategies for preterm labor in the setting of infection. We developed the first experimental model in pregnant rhesus monkeys in which intrauterine infection is established by the intraamniotic inoculation of known concentrations of Group B streptococcus (GBS) bacteria. Following inoculation, there is sequential production of the proinflammmatory cytokines, prostaglandins, and matrix metalloproteinases (MMPs). This is then followed in all cases by uterine contraction, labor and delivery, usually within 72 hours.