SC COBRE: LIPIDOMICS CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 生物活性脂质的多样性及其相互关联的代谢提供了调节细胞内和细胞间信号传导和功能的途径网络。 这些通路的功能障碍导致特定疾病的病理生物学，如癌症进展和转移、加速衰老、炎症和真菌发病机制。 这强调了发展脂质化学和分析的需要。 脂质组学核心是基于脂质化学，分析和生物学关键人员的独特专业知识创建的，已经发展成为一个机构，国家甚至国际资源，满足研究界在鞘脂领域的需求。 核心课程提供脂质学各个方面的概念和实践培训，从不同生物材料中对脂质成分进行定性和定量分析（细胞、组织、生物体液），研究脂质代谢的合成分子工具（功能化和荧光神经酰胺，位点特异性放射性鞘脂），用于细胞，体外，和体内研究（细胞器靶向鞘脂和鞘脂代谢酶的细胞器靶向抑制剂），并协助研究人员进行实验设计，选择感兴趣的脂质和解释分析结果。 分析方法基于高效液相色谱-串联质谱（LC-MS）技术。 目前，我们提供鞘氨醇碱及其磷酸盐、神经酰胺及其磷酸盐、鞘磷脂和甘油二酯的同时分析。 葡萄糖神经酰胺处于最后的开发阶段。 我们的目标是获得鞘脂的代谢组学特征。 该核心一直有助于COBRE PI的成功，并获得两个计划项目赠款的校外资金，并获得NIH的竞争性共享仪器赠款，这支持了我们的第二个MS仪器。 该中心参与了几个合作项目，并提供有偿服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The diversity ofr bioactive lipids and their interconnected metabolism provides a network of pathways regulating intra- and inter-cellular signaling and function. Dysfunction in these pathways contribute to the pathobiology of specific diseases such as cancer progression and metastasis, accelerated aging, inflammation, and fungal pathogenesis. This emphasized needs for developing lipid chemistry and analysis. The Lipidomics Core was created based on unique expertise of the key personnel in lipid chemistry, analysis and biology has evolved into an institutional, national, and even international resource that serves the needs of the research community in the field of Sphinglipids. The core provides conceptual and practical training in various aspects of lipidology, qualitative and quantitative analysis of lipid components from different biological materials (cells, tissue, biological fluids), synthetic molecular tools to study lipid metabolism (funtionalized and fluorescent ceramides, site-specific radioactive sphingolipids), diversified synthetic lipids and analogs for cellular, in vitro, and in vivo studies (organelle-targeting sphingolipids and organelle-targeting inhibitors of sphingolipid metabolizing enzymes), and assists investigators in experimental design, selection of lipid of interest and interpretation of the analytical results. Analytical approach is based on High Performance Liquid Chromatography-Tandem Mass Spectrometry (LC-MS) technology. Currently, we provide simultaneous analysis of sphingoid bases and their phosphates, ceramide species and their phosphates, sphingomyelin species and diacyl-glycerol species. Glucosylceramide is under a final developmental stage. Our goal is to reach metabolomic profile of sphingolipids. The core has been instrumental to the success of the COBRE PIs and in obtaining extramural funding for the two Program Project Grants and the award of a competitive shared instrument grant from NIH, which supported our second MS instrument. The core is engaged in several collaborative projects and provides also a paid fee-for-service.