SC COBRE: LIPIDOMICS CORE

SC COBRE：脂质组学核心

• 批准号: 7959962
• 负责人: ALICJA BIELAWSKA
• 金额: $ 43.8万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959962
• 关键词: Aging; Award; Biocompatible Materials; Biological; Biology; Cells; Centers of Research Excellence; Ceramides; Communities; Computer Retrieval of Information on Scientific Projects Database; Development; Disease; Enzymes; Experimental Designs; Extramural Activities; Fee-for-Service Plans; Functional disorder; Funding; Glucosylceramides; Glycerol; Goals; Grant; High Pressure Liquid Chromatography; Human Resources; In Vitro; Inflammation; Institution; International; Lipid Chemistry; Lipids; Liquid substance; Metabolism; Molecular; Neoplasm Metastasis; Organelles; Pathogenesis; Pathway interactions; Program Research Project Grants; Radioactive; Research; Research Personnel; Resources; Signal Transduction; Site; Source; Sphingolipids; Sphingomyelins; Staging; Technology; Tissues; Training; United States National Institutes of Health; analog; base; in vivo; inhibitor/antagonist; inorganic phosphate; instrument; interest; lipid metabolism; metabolomics; success; tandem mass spectrometry; tool; tumor progression

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The diversity ofr bioactive lipids and their interconnected metabolism provides a network of pathways regulating intra- and inter-cellular signaling and function. Dysfunction in these pathways contribute to the pathobiology of specific diseases such as cancer progression and metastasis, accelerated aging, inflammation, and fungal pathogenesis. This emphasized needs for developing lipid chemistry and analysis. The Lipidomics Core was created based on unique expertise of the key personnel in lipid chemistry, analysis and biology has evolved into an institutional, national, and even international resource that serves the needs of the research community in the field of Sphinglipids. The core provides conceptual and practical training in various aspects of lipidology, qualitative and quantitative analysis of lipid components from different biological materials (cells, tissue, biological fluids), synthetic molecular tools to study lipid metabolism (funtionalized and fluorescent ceramides, site-specific radioactive sphingolipids), diversified synthetic lipids and analogs for cellular, in vitro, and in vivo studies (organelle-targeting sphingolipids and organelle-targeting inhibitors of sphingolipid metabolizing enzymes), and assists investigators in experimental design, selection of lipid of interest and interpretation of the analytical results. Analytical approach is based on High Performance Liquid Chromatography-Tandem Mass Spectrometry (LC-MS) technology. Currently, we provide simultaneous analysis of sphingoid bases and their phosphates, ceramide species and their phosphates, sphingomyelin species and diacyl-glycerol species. Glucosylceramide is under a final developmental stage. Our goal is to reach metabolomic profile of sphingolipids. The core has been instrumental to the success of the COBRE PIs and in obtaining extramural funding for the two Program Project Grants and the award of a competitive shared instrument grant from NIH, which supported our second MS instrument. The core is engaged in several collaborative projects and provides also a paid fee-for-service.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 生物活性脂质的多样性及其相互关联的代谢提供了调节细胞内和细胞间信号传导和功能的途径网络。 这些途径的功能障碍会导致特定疾病的病理生物学，例如癌症进展和转移、加速衰老、炎症和真菌发病机制。 这强调了开发脂质化学和分析的需求。 脂质组学核心是基于脂质化学、分析和生物学领域关键人员的独特专业知识而创建的，已发展成为一个机构、国家甚至国际资源，满足鞘脂领域研究界的需求。 该核心课程提供脂质学各个方面的概念和实践培训，对来自不同生物材料（细胞、组织、生物液体）的脂质成分进行定性和定量分析，研究脂质代谢的合成分子工具（功能化和荧光神经酰胺，位点特异性放射性鞘脂），用于细胞、体外和体外的多样化合成脂质和类似物。 体内研究（细胞器靶向鞘脂和细胞器靶向鞘脂代谢酶抑制剂），并协助研究人员进行实验设计、感兴趣脂质的选择和分析结果的解释。 分析方法基于高效液相色谱-串联质谱 (LC-MS) 技术。 目前，我们提供鞘氨醇碱及其磷酸盐、神经酰胺种类及其磷酸盐、鞘磷脂种类和二酰基甘油种类的同步分析。 葡萄糖神经酰胺正处于最后的开发阶段。 我们的目标是获得鞘脂的代谢组学特征。 该核心对于 COBRE PI 的成功以及为两个计划项目拨款获得外部资金以及从 NIH 授予竞争性共享仪器拨款（支持我们的第二台 MS 仪器）发挥了重要作用。 该核心参与了多个合作项目，并提供付费服务。