Core A: Lipidomics

总结 PPG中脂质组学核心的目的是提供智力和体力资源（特别是 鞘脂和合成分子工具的最新质谱分析），以增强 了解生物活性脂质在癌症生物学中的作用，目的是发现未来的治疗方法。的 生物活性脂质的多样性及其相互关联的代谢产生了一个调节途径网络， 细胞内和细胞间的信号传导和功能。这些途径的功能障碍导致了 癌症进展和转移。这些考虑使脂质的发展成为必要。 化学和分析。脂质组学核心是基于以下关键人员的独特专业知识创建的： 脂质化学、分析和代谢。该核心已发展成为一个机构性、国家性和 脂质组学和脂质化学生物学新兴领域的国际资源。核心服务包括：1） 提供来自不同生物材料（细胞， 组织和生物流体），主要采用高效液相色谱-串联质谱 质谱（HPLC-MS/MS）技术-核心目前提供了300多个定量分析 在基础代谢组学水平上的不同脂质分子种类; 2）开发和提供合成分子 研究脂质代谢的工具（例如，功能化和荧光神经酰胺，特定位点放射性 鞘脂，17 C-鞘脂）和用于细胞、体外和体内的多种合成脂质和类似物 研究（例如，靶向细胞器的鞘脂和靶向细胞器的鞘脂代谢抑制剂 3）提供直接MALDI-MS组织分析以可视化基于脂质的抗癌药物的分布 药物及其对鞘脂成分的分子效应;以及4）协助PPG研究人员 实验设计、目标脂质的选择和分析结果的解释。

SUMMARY The purpose of the Lipidomics Core in the PPG is to provide intellectual and physical resources (particularly state-of-the-art mass spectrometry analysis of sphingolipids and synthetic molecular tools) to enhance understanding of the role of bioactive lipids in cancer biology with the goal to discover future therapeutics. The diversity of bioactive lipids and their interconnected metabolism generates a network of pathways regulating intra- and inter-cellular signaling and function. Dysfunctions in these pathways contribute to the pathobiology of cancer progression and metastasis. These considerations have necessitated the development of lipid chemistry and analysis. The Lipidomics Core was created based on unique expertise of the key personnel in lipid chemistry, analysis, and metabolism. This Core has developed into an institutional, national, and international resource in the emerging fields of lipidomics and lipid chemical biology. Core services include: 1) Providing qualitative and quantitative analysis of lipid components from different biological materials (cells, tissue, and biological fluids), primarily employing high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technology—the Core currently provides quantitative analysis of more than 300 distinct lipid molecular species at a basic metabolomic level; 2) Developing and providing synthetic molecular tools to study lipid metabolism (e.g., functionalized and fluorescent ceramides, site-specific radioactive sphingolipids, 17C-sphingolipids) and diversified synthetic lipids and analogs for cellular, in vitro, and in vivo studies (e.g., organelle-targeted sphingolipids and organelle-targeted inhibitors of sphingolipid metabolizing enzymes); 3) Providing direct MALDI-MS tissue analysis to visualize the distribution of lipid based anti-cancer drugs and their molecular effects on sphingolipid components; and 4) Assisting PPG investigators in experimental design, selection of lipids of interest, and interpretation of analytical results.